Browse

Lipid products of phosphoinositide 3-kinase abrogate genistein-induced fusion inhibition in myoblasts

DC Field Value Language
dc.contributor.authorWoo, Joo Hong-
dc.contributor.authorKim, Jeong Heon-
dc.contributor.authorMook-Jung, Inhee-
dc.contributor.authorKim, Hye Sun-
dc.date.accessioned2010-01-04T05:02:50Z-
dc.date.available2010-01-04T05:02:50Z-
dc.date.issued2006-
dc.identifier.citationEur. J. Pharmacol. 529, 84-94en
dc.identifier.issn0014-2999 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16360147-
dc.identifier.urihttp://hdl.handle.net/10371/24862-
dc.description.abstractGenistein (4',5,7-trihydroxyisoflavone) is a tyrosine kinase inhibitor. Although the agent has shown to inhibit myoblast differentiation, neither intracellular target(s) as a tyrosine kinase inhibitor nor action mechanism of the agent is well known. Here we studied the effect of genistein on the differentiation of myoblasts. Genistein strongly but reversibly blocked both myoblast fusion and synthesis of the muscle-specific proteins. The agent also reversibly reduced the phosphorylation level of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase, and its interaction with p85, the regulatory subunit of phosphoinositide 3-kinase (PI3-kinase). In addition, genistein indirectly inhibited PI3-kinase activity and blocked calcium influx which is required for myoblast fusion. However, both genistein-induced inhibition of cell fusion and calcium influx were abrogated by the lipid products of PI3-kinase. These results demonstrate that genistein can exert their effect on the signaling pathway from FAK to calcium influx via PI3-kinase in the differentiation of myoblasts.en
dc.language.isoenen
dc.publisherElsevieren
dc.subject1-Phosphatidylinositol 3-Kinase/*antagonists & inhibitors/metabolismen
dc.subjectAnimalsen
dc.subjectCalcium/metabolismen
dc.subjectCell Differentiation/*drug effectsen
dc.subjectCell Fusionen
dc.subjectCells, Cultureden
dc.subjectEnzyme Inhibitors/*pharmacologyen
dc.subjectFocal Adhesion Protein-Tyrosine Kinases/metabolismen
dc.subjectGenistein/*pharmacologyen
dc.subjectLipids/*pharmacologyen
dc.subjectMyoblasts, Skeletal/cytology/*drug effectsen
dc.subjectPhosphorylationen
dc.subjectRatsen
dc.titleLipid products of phosphoinositide 3-kinase abrogate genistein-induced fusion inhibition in myoblastsen
dc.typeArticleen
dc.contributor.AlternativeAuthor우주홍-
dc.contributor.AlternativeAuthor김정헌-
dc.contributor.AlternativeAuthor묵정인희-
dc.contributor.AlternativeAuthor김혜선-
dc.identifier.doi10.1016/j.ejphar.2005.11.007-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse