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p21WAF/CIP1/SDI1 is upregulated due to increased mRNA stability during hydroxyurea-induced senescence of human fibroblasts

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dc.contributor.authorKim, Hyun-Seok-
dc.contributor.authorYeo, Eui-ju-
dc.contributor.authorPark, Seong-Hoon-
dc.contributor.authorPark, Joo-In-
dc.contributor.authorPark, Sang-Chul-
dc.contributor.authorShin, Jong-Yeon-
dc.contributor.authorKim, Min-Ju-
dc.contributor.authorOh, Soo-Jin-
dc.contributor.authorWon, Moo-Ho-
dc.contributor.authorKang, Tae-Chun-
dc.contributor.authorPark, Jae-Bong-
dc.contributor.authorKim, Jaebong-
dc.contributor.authorKim, Jong-Il-
dc.contributor.authorLee, Hyun-Yong-
dc.contributor.authorLee, Jae-Yong-
dc.date.accessioned2010-01-06T05:00:04Z-
dc.date.available2010-01-06T05:00:04Z-
dc.date.issued2005-08-23-
dc.identifier.citationMech Ageing Dev. 2005 Dec;126(12):1255-61. Epub 2005 Aug 18.en
dc.identifier.issn0047-6374 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16111738-
dc.identifier.urihttps://hdl.handle.net/10371/26283-
dc.description.abstractHydoxyurea induces senescence-like growth arrest in normal human fibroblasts. p21(WAF/CIP1/SDI1), a cyclin dependent kinase inhibitor, was found to be upregulated during this growth arrest. Levels of p21(WAF/CIP1/SDI1) protein and mRNA were increased nine-fold by hydroxyurea in these cells. In order to determine whether p21(WAF/CIP1/SDI1) mRNA is increased by hydroxyurea at the transcriptional level, human fibroblast cells were transfected with reporter constructs containing a p21(WAF/CIP1/SDI1) promoter fragment and then treated with hydroxyurea. The luciferase activities in the reporter-transfected fibroblast cells were not increased by hydroxyurea, indicating that p21(WAF/CIP1/SDI1) transcription was not elevated by hydroxyurea. The half-life of the p21(WAF/CIP1/SDI1) mRNA was increased by 2.5-fold but that of p21(WAF/CIP1/SDI1) protein was not. Our results suggest that increased mRNA stability is the major mechanism of p21(WAF/CIP1/SDI1) elevation in the hydroxyurea-induced growth arrest of human fibroblasts.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectBlotting, Northernen
dc.subjectBlotting, Westernen
dc.subjectCell Agingen
dc.subjectCells, Cultureden
dc.subjectCyclin-Dependent Kinase Inhibitor p21/*biosynthesisen
dc.subjectDose-Response Relationship, Drugen
dc.subjectFibroblasts/*cytology/metabolismen
dc.subject*Gene Expression Regulationen
dc.subjectGenes, Reporteren
dc.subjectHumansen
dc.subjectHydroxyurea/metabolism/*pharmacologyen
dc.subjectLuciferases/metabolismen
dc.subjectNucleic Acid Conformationen
dc.subjectPlasmids/metabolismen
dc.subjectPromoter Regions, Geneticen
dc.subjectRNA, Messenger/*metabolismen
dc.subjectTime Factorsen
dc.subjectTranscription, Geneticen
dc.subjectTransfectionen
dc.subject*Up-Regulationen
dc.titlep21WAF/CIP1/SDI1 is upregulated due to increased mRNA stability during hydroxyurea-induced senescence of human fibroblastsen
dc.typeArticleen
dc.contributor.AlternativeAuthor김현석-
dc.contributor.AlternativeAuthor여의주-
dc.contributor.AlternativeAuthor박성훈-
dc.contributor.AlternativeAuthor박주인-
dc.contributor.AlternativeAuthor박상철-
dc.contributor.AlternativeAuthor신정연-
dc.contributor.AlternativeAuthor김민주-
dc.contributor.AlternativeAuthor오수진-
dc.contributor.AlternativeAuthor원무호-
dc.contributor.AlternativeAuthor강태천-
dc.contributor.AlternativeAuthor박재봉-
dc.contributor.AlternativeAuthor김재봉-
dc.contributor.AlternativeAuthor김종일-
dc.contributor.AlternativeAuthor이현용-
dc.contributor.AlternativeAuthor이재용-
dc.identifier.doi10.1016/j.mad.2005.07.002-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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