Publications

Detailed Information

The analysis of telomere length and telomerase activity in cloned pigs and cows

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors

Jeon, H Y; Hyun, S H; Lee, G S; Kim, H S; Kim, S; Jeong, Y W; Kang, S K; Lee, B C; Han, J Y; Ahn, C; Hwang, W S

Issue Date
2005-04-05
Publisher
Wiley-Blackwell
Citation
Mol Reprod Dev. 2005 Jul;71(3):315-20.
Keywords
AnimalsBlastocyst/*metabolismCattleGene Expression Regulation, Developmental/physiologySwineTelomerase/*metabolismTelomere/*metabolismUp-Regulation/physiologyCloning, Organism
Abstract
Inefficiency in the production of cloned animals is most likely due to epigenetic reprogramming errors after somatic cell nuclear transfer (SCNT). In order to investigate whether nuclear reprogramming restores cellular age of donor cells after SCNT, we measured telomere length and telomerase activity in cloned pigs and cattle. In normal pigs and cattle, the mean telomere length was decreased with biological aging. In cloned or transgenic cloned piglets, the mean telomere length was elongated compared to nuclear donor fetal fibroblasts and age-matched normal piglets. In cloned cattle, no increases in mean telomere length were observed compared to nuclear donor adult fibroblasts. In terms of telomerase activity, significant activity was observed in nuclear donor cells and normal tissues from adult or new-born pigs and cattle, with relatively higher activity in the porcine tissues compared to the bovine tissues. Cloned calves and piglets showed the same level of telomerase activity as their respective donor cells. In addition, no difference in telomerase activity was observed between normal and transgenic cloned piglets. However, increased telomerase activity was observed in porcine SCNT blastocysts compared to nuclear donor cells and in vitro fertilization (IVF)-derived blastocysts, suggesting that the elongation of telomere lengths observed in cloned piglets could be due to the presence of higher telomerase activity in SCNT blastocysts. In conclusion, gathering from the comparative studies with cattle, we were able to demonstrate that telomere length in cloned piglets was rebuilt or elongated with the use of cultured donor fetal fibroblasts.
ISSN
1040-452X (Print)
http://dx.doi.org/10.1002/mrd.20279
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15806556

https://hdl.handle.net/10371/26838
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share