Browse

ER stress-induced caspase-12 activation is inhibited by PKC in neuronal cells

Cited 15 time in Web of Science Cited 16 time in Scopus
Authors
Lee, W.; Kim, D. H.; Boo, J. H.; Kim, Y. H.; Park, I. S.; Mook-Jung, I.
Issue Date
2005-04-22
Publisher
Springer Verlag
Citation
Apoptosis. 2005 Mar;10(2):407-15.
Keywords
AnimalsBlotting, WesternCaspase 12Caspase 3Caspases/*metabolismCell LineCytoplasm/metabolismEndoplasmic Reticulum/*metabolismEnzyme ActivationImmunohistochemistryNeurons/enzymology/*metabolismPhorbol 12,13-Dibutyrate/pharmacologyProtein Kinase C/*metabolismProtein Structure, TertiaryProtein TransportRatsRecombinant Proteins/chemistrySignal TransductionThapsigargin/pharmacologyTime Factors
Abstract
Caspase-12 is activated when the cells are exposed to excess levels of various stimuli, which cause endoplasmic reticulum (ER) stress. Protein kinase C (PKC) plays an important role in many signaling pathways in cells, and the activation of PKC has multiple actions in the signaling function of the ER. This study examined whether or not phorbol 12, 13-dibutyrate (PDBu)-induced PKC activation modulates caspase-12 cleavage and it's processing, using a wild type caspase-12 overexpressing neuronal cell line, known as Cas-12 cells. The thapsigargin treatment induced caspase-12 fragmentation in the Cas-12 cells. This was inhibited by PKC, which had previously been stimulated by PDBu. The PDBu treatment attenuated the ER stress-induced translocation of caspase-12 from the ER to the cytoplasm. The caspase-3 specific inhibitor blocked caspase-12 fragmentation, and purified caspase-12 was cleaved by the active caspase-3 in vitro, suggesting that caspase-12 might be a substrate for caspase-3. In addition, the PDBu treatment influenced the decrease of active caspase-3 fragment. These results suggest that an ER stress induces the activation of caspase-12 via caspase-3, and that PKC regulates both caspase-12 and caspase-3 activations in Cas-12 cells.
ISSN
1360-8185 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15843901

https://hdl.handle.net/10371/26890
DOI
https://doi.org/10.1007/s10495-005-0814-6
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse