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Zinc-fingers and homeoboxes 1 (ZHX1) binds DNA methyltransferase (DNMT) 3B to enhance DNMT3B-mediated transcriptional repression

Cited 29 time in Web of Science Cited 33 time in Scopus
Authors
Kim, Sung-Hak; Park, Jinah; Choi, Moon-Chang; Kim, Hwang-Phill; Park, Jung-Hyun; Jung, Yeonjoo; Lee, Ju-Hee; Oh, Do-Youn; Im, Seock-Ah; Bang, Yung-Jue; Kim, Tae-You
Issue Date
2007-04
Publisher
Elsevier
Citation
Biochemical and Biophysical Research Communications, Vol.355 No.2, pp.318-323
Keywords
DNMT3BZHX1yeast two-hybridprotein-protein interactiontranscriptional repression
Abstract
DNA methyltransferases (DNMT) 313 is a de novo DNMT that represses transcription independent of DNMT activity. In order to gain a better insight into DNMTB3-mediated transcriptional repression, we performed a yeast two-hybrid analysis using DNMT3B as a bait. Of the various binding candidates, ZHX1, a member of zinc-finger and homeobox protein, was found to interact with DNMT3B in vivo and in vitro. N-terminal PWWP domain of DNMT3B was required for its interaction with homeobox motifs of ZHX1. ZHX1 contains nuclear localization signal at C-terminal homeobox motif, and both ZHX1 and DNMT3B were co-localized in nucleus. Furthermore, we found that ZHX1 enhanced the transcriptional repression mediated by DNMT3B when DNMT3B is directly targeted to DNA. These results showed for the first the direct linkage between DNMT and zinc-fingers homeoboxes protein, leading to enhanced gene silencing by DNMT3B. (c) 2007 Elsevier Inc. All rights reserved.
ISSN
0006-291X
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17303076

https://hdl.handle.net/10371/26983
DOI
https://doi.org/10.1016/j.bbrc.2007.01.187
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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