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Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells

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dc.contributor.authorCho, Young Min-
dc.contributor.authorLim, Jung Mee-
dc.contributor.authorYoo, Dae Hoon-
dc.contributor.authorKim, Jae Hyeon-
dc.contributor.authorChung, Sung Soo-
dc.contributor.authorPark, Sang Gyu-
dc.contributor.authorKim, Tae Hyuk-
dc.contributor.authorOh, Sun Kyung-
dc.contributor.authorChoi, Young Min-
dc.contributor.authorMoon, Shin Yong-
dc.contributor.authorPark, Kyong Soo-
dc.contributor.authorLee, Hong Kyu-
dc.date.accessioned2010-01-06T23:40:48Z-
dc.date.available2010-01-06T23:40:48Z-
dc.date.issued2007-
dc.identifier.citationBiochemical and Biophysical Research Communications 366 (2008) 129-134en
dc.identifier.issn1090-2104 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18060872-
dc.identifier.urihttps://hdl.handle.net/10371/27167-
dc.description.abstractThe major obstacle in cell therapy of diabetes mellitus is the limited source of insulin-producing beta cells. Very recently, it was shown that a five-stage protocol recapitulating in vivo pancreatic organogenesis induced pancreatic beta cells in vitro; however, this protocol is specific to certain cell lines and shows much line-to-line variation in differentiation efficacy. Here, we modified the five-stage protocol for the human embryonic stem cell line SNUhES3 by the addition of betacellulin and nicotinamide. We reproduced in vivo pancreatic islet differentiation by directing the cells through stages that resembled in vivo pancreatic organogenesis. The addition of betacellulin and nicotinamide sustained PDX1 expression and induced beta-cell differentiation. C-peptide-a genuine marker of de novo insulin production-was identified in the differentiated cells, although the insulin mRNA content was very low. Further studies are necessary to develop more efficient and universal protocols for beta-cell differentiation.en
dc.description.sponsorshipThis research was supported by grants (SC2020 and
SC1020) from Stem Cell Research Center of the 21st Century
Frontier Research Program funded by the Ministry of
Science and Technology, Republic of Korea.
en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectCell Differentiation/drug effectsen
dc.subjectCell Lineen
dc.subjectDose-Response Relationship, Drugen
dc.subjectEmbryonic Stem Cells/*cytology/drug effects/*physiologyen
dc.subjectGene Expression Regulation, Developmental/drug effects/physiologyen
dc.subjectHomeodomain Proteins/*metabolismen
dc.subjectHumansen
dc.subjectInsulin-Secreting Cells/*cytology/drug effects/*physiologyen
dc.subjectIntercellular Signaling Peptides and Proteins/*administration & dosageen
dc.subjectNiacinamide/*administration & dosageen
dc.subjectTrans-Activators/*metabolismen
dc.titleBetacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor조영민-
dc.contributor.AlternativeAuthor임정미-
dc.contributor.AlternativeAuthor유대훈-
dc.contributor.AlternativeAuthor김재현-
dc.contributor.AlternativeAuthor정성수-
dc.contributor.AlternativeAuthor박상규-
dc.contributor.AlternativeAuthor김태혁-
dc.contributor.AlternativeAuthor오선경-
dc.contributor.AlternativeAuthor최영민-
dc.contributor.AlternativeAuthor문신용-
dc.contributor.AlternativeAuthor박경수-
dc.contributor.AlternativeAuthor이홍규-
dc.identifier.doi10.1016/j.bbrc.2007.11.112-
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