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Voltage-dependent slowly activating anion current regulated by temperature and extracellular pH in mouse B cells

Cited 3 time in Web of Science Cited 3 time in Scopus
Authors
Nam, Joo Hyun; Zheng, Hai Feng; Earm, Ki Hyun; Ko, Jae Hong; Lee, Ik-Jae; Kang, Tong Mook; Kim, Tae Jin; Earm, Young E.; Kim, Sung Joon
Issue Date
2006-06-08
Publisher
Springer Verlag
Citation
Pflugers Arch. 2006 Sep;452(6):707-17. Epub 2006 Jun 7.
Keywords
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacologyAdenosine Triphosphate/metabolismAnimalsAnti-Inflammatory Agents, Non-Steroidal/pharmacologyB-Lymphocytes/*physiologyCell LineElectrophysiologyHydrogen-Ion ConcentrationIon Channels/*physiologyKineticsMembrane Potentials/physiologyMicePatch-Clamp TechniquesNiflumic Acid/pharmacologyTemperature
Abstract
Voltage-dependent, outwardly rectifying anion channels have been described in various cells including lymphocytes. In this study, we found that murine B cells express the voltage-dependent slowly activating anion channels (VSACs). Using a whole-cell configuration, I (VSAC) in Bal-17 was induced by a sustained depolarization (>0 mV) which was remarkably facilitated at 35 degrees C (Q (10)=23 at 30 mV of clamp voltage). Substitution of extracellular Cl(-) with gluconate shifted the reversal potential to the right (35.7 mV). Gd(3+) (IC(50)=0.11 microM) significantly attenuated I (VSAC), but DIDS partially blocked I (VSAC). In addition, extracellular acidification suppressed I (VSAC) whereas alkalinization facilitated the channel activation. I (VSAC) was decreased by 90% at pH 6.35 and increased by 180% at pH 8.0. In cell-attached and inside-out patch clamps, depolarization slowly activated the anion channels of large conductance (approximately 270 pS) with multiple levels of subconductances. The single channel currents were also blocked by Gd(3+) and acidic pH. Furthermore, I (VSAC) was also observed in WEHI-231 (an immature B cell line) and freshly isolated splenic B cells of mice. In summary, murine B cells express unique voltage-dependent anion channels that show a strong sensitivity to both temperature and extracellular pH. Further investigation is required to understand the physiological roles of VSAC and its molecular identity.
ISSN
0031-6768 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16758225

https://hdl.handle.net/10371/27470
DOI
https://doi.org/10.1007/s00424-006-0084-3
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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