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In vivo monitoring of DNA vaccine gene expression using firefly luciferase as a naked DNA
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, Yong Hyun | - |
dc.contributor.author | Choi, Yun | - |
dc.contributor.author | Kang, Joo Hyun | - |
dc.contributor.author | Chung, June-Key | - |
dc.contributor.author | Lee, Yong Jin | - |
dc.contributor.author | Kim, Chul Woo | - |
dc.contributor.author | Jeong, Jae Min | - |
dc.contributor.author | Lee, Dong Soo | - |
dc.contributor.author | Lee, Myung Chul | - |
dc.date.accessioned | 2010-01-07T01:10:55Z | - |
dc.date.available | 2010-01-07T01:10:55Z | - |
dc.date.issued | 2006-02-18 | - |
dc.identifier.citation | Vaccine. 2006 Apr 12;24(16):3057-62. Epub 2006 Jan 30. | en |
dc.identifier.issn | 0264-410X (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16483696 | - |
dc.identifier.uri | https://hdl.handle.net/10371/27567 | - |
dc.description.abstract | The administration of naked DNA into animals is increasing as a research tool to develop DNA vaccine. To monitor the distribution and duration of gene expression of a DNA vaccine in living organisms, we used the naked DNA encoding firefly luciferase (Fluc) as an imaging reporter gene, and evaluated in vivo bioluminescent images in a murine model. We observed bioluminescence at the injection site and at inguinal lymph node from 10h to 24h post-injection when DNA vaccine encoding Fluc (pcDNA3.1-Fluc) was injected into the bilateral posterior flanks in mice. Fluc gene expressions at injection sites and unilateral posterior flank inguinal lymph node were also confirmed by RT-PCR. However, when pcDNA3.1-Fluc was injected into the mid-dorsum bioluminescent signals were observed at the injection site for up to 14 days post-injection, but no bioluminescent signals were detected in inguinal lymph nodes. Concurrent mRNA expressions of Fluc gene at injection sites but not at inguinal lymph nodes were confirmed by RT-PCR. These findings suggest that optical imaging using Fluc could be useful for monitoring the location, intensity and duration of gene expression of naked DNA vaccines in living animals non-invasively and repetitively. | en |
dc.language.iso | en | - |
dc.publisher | Elsevier | en |
dc.subject | Animals | en |
dc.subject | Female | en |
dc.subject | Genes, Reporter | en |
dc.subject | Image Processing, Computer-Assisted | en |
dc.subject | Luciferases, Firefly/*analysis/genetics | en |
dc.subject | Luminescent Measurements | en |
dc.subject | Lymph Nodes/immunology | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred BALB C | en |
dc.subject | Models, Animal | en |
dc.subject | RNA, Messenger/analysis | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | Vaccines, DNA/administration & dosage/*genetics/*immunology | en |
dc.subject | Gene Expression | - |
dc.title | In vivo monitoring of DNA vaccine gene expression using firefly luciferase as a naked DNA | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 전용현 | - |
dc.contributor.AlternativeAuthor | 최윤 | - |
dc.contributor.AlternativeAuthor | 강주현 | - |
dc.contributor.AlternativeAuthor | 정준기 | - |
dc.contributor.AlternativeAuthor | 이용진 | - |
dc.contributor.AlternativeAuthor | 김철우 | - |
dc.contributor.AlternativeAuthor | 정재민 | - |
dc.contributor.AlternativeAuthor | 이동수 | - |
dc.contributor.AlternativeAuthor | 이명철 | - |
dc.identifier.doi | 10.1016/j.vaccine.2006.01.033 | - |
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