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Different roles of histone H3 lysine 4 methylation in chromatin maintenance

Cited 7 time in Web of Science Cited 7 time in Scopus
Authors
Seol, Ja-Hwan; Kim, Hye-Jin; Yang, Yong-Jin; Kim, Seong-Tae; Youn, Hong-Duk; Han, Jeong-Whan; Lee, Hyang-Woo; Cho, Eun-Jung
Issue Date
2006-09-09
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2006 Oct 20;349(2):463-70. Epub 2006 Aug 31.
Keywords
Cell Cycle Proteins/metabolismChromatin/*chemistryDNA-Binding Proteins/physiologyEpigenesis, GeneticFungal Proteins/chemistryHeterochromatin/chemistryHistone-Lysine N-MethyltransferaseHistones/*chemistryLysine/*chemistryMethyltransferases/physiologyMutagenesisNuclear Proteins/physiologyProtein Structure, TertiaryProtein-Serine-Threonine Kinases/metabolismSaccharomyces cerevisiae/genetics/*metabolismSaccharomyces cerevisiae Proteins/metabolism/physiologyTranscription Factors/physiology
Abstract
Histone H3 methyltransferases are involved in the epigenetic control of transcription and heterochromatin maintenance. In Saccharomyces cerevisiae, deletion of a histone H3 methyltransferase SET1 leads to the induction of a subset of stress responsive genes in a Rad53 dependent manner. This type of induction was observed only in the absence of SET1 and not in the absence of other histone methyltransferases, SET2 or DOT1. We show that the increased expression of the stress responsive genes results from a lack of histone H3 lysine (K) 4 methylation. The loss of mono-methylation on H3 K4 is necessary to increase the expression of the stress responsive genes, while the loss of di- or tri-methylation induced by deletion of either RRM domain of Set1 or the upstream effector molecules hardly affected their expression. These results suggest that mono- and multiple methylation of H3 K4 have different roles. The mono-methylation of H3 K4 might be required for the global integrity of chromatin structure, which is normally monitored by the Rad53 dependent chromatin surveillance system.
ISSN
0006-291X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16959218

http://hdl.handle.net/10371/27604
DOI
https://doi.org/10.1016/j.bbrc.2006.08.122
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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