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Improvement of postnatal neovascularization by human embryonic stem cell derived endothelial-like cell transplantation in a mouse model of hindlimb ischemia
DC Field | Value | Language |
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dc.contributor.author | Moon, Sung-Hwan | - |
dc.contributor.author | Lee, Soo-Hong | - |
dc.contributor.author | Kang, Sun-Woong | - |
dc.contributor.author | Kim, Jumi | - |
dc.contributor.author | Lim, Jae Min | - |
dc.contributor.author | Kim, Hyo-Soo | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.contributor.author | Chung, Hyung-Min | - |
dc.contributor.author | Cho, Seung-Woo | - |
dc.date.accessioned | 2010-01-07T05:24:51Z | - |
dc.date.available | 2010-01-07T05:24:51Z | - |
dc.date.issued | 2007-11-20 | - |
dc.identifier.citation | Circulation. 2007;116:2409-2419 | en |
dc.identifier.issn | 1524-4539 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17984381 | - |
dc.identifier.uri | https://hdl.handle.net/10371/28279 | - |
dc.description.abstract | BACKGROUND: We established an efficient preparation method to obtain endothelial-like cells (ECs) from human embryonic stem cells (hESCs) and tested whether these hESC-ECs would show therapeutic potential for treatment of hindlimb ischemia. METHODS AND RESULTS: ECs differentiated from hESCs were obtained by mechanical isolation and cell sorting for von Willebrand factor. The isolated hESC-ECs maintained endothelial cell-specific characteristics such as endothelial marker expression and capillary formation. One day after surgical induction of hindlimb ischemia in athymic mice, hESC-ECs were injected intramuscularly into ischemic limbs. Four weeks after treatment, hESC-EC treatment significantly increased limb salvage (36%) compared with treatment with medium (0%). In addition, laser Doppler imaging showed that the ratio of blood perfusion (ischemic to normal limb) was increased significantly (P<0.01) by hESC-EC treatment (0.511+/-0.167) compared with medium injection (0.073+/-0.061). Capillary and arteriole densities were 658+/-190/mm2 and 30+/-11/mm2 in the hESC-EC group, respectively, whereas those in the medium group were 392+/-118/mm2 and 16+/-8/mm2, respectively (P<0.01). Reverse-transcription polymerase chain reaction with human-specific primers revealed mRNA expression of human endothelial markers and human angiogenic factors in ischemic mouse tissues. The transplanted hESC-ECs were localized as capillaries near muscle tissues in ischemic regions or incorporated in the vessels between muscle tissues, as confirmed by human nuclear antigen staining with platelet/endothelial cell adhesion molecule or von Willebrand factor. CONCLUSIONS: This study demonstrates that hESC-EC transplantation improves blood perfusion and limb salvage by facilitating postnatal neovascularization in a mouse model of hindlimb ischemia. Thus, hESC-ECs might be useful as an alternative cell source for angiogenic therapy. | en |
dc.language.iso | en | en |
dc.publisher | American Heart Association | en |
dc.subject | Animals | en |
dc.subject | Animals, Newborn | en |
dc.subject | Cell Line | en |
dc.subject | Disease Models, Animal | en |
dc.subject | Embryonic Stem Cells/*transplantation | en |
dc.subject | Endothelial Cells/*transplantation | en |
dc.subject | Female | en |
dc.subject | Hindlimb/*blood supply/surgery | en |
dc.subject | Humans | en |
dc.subject | Ischemia/pathology/*physiopathology/*surgery | en |
dc.subject | Mice | en |
dc.subject | Mice, Nude | en |
dc.subject | Neovascularization, Physiologic/*physiology | en |
dc.subject | Stem Cell Transplantation/methods | en |
dc.title | Improvement of postnatal neovascularization by human embryonic stem cell derived endothelial-like cell transplantation in a mouse model of hindlimb ischemia | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 조승우 | - |
dc.contributor.AlternativeAuthor | 문성환 | - |
dc.contributor.AlternativeAuthor | 이수홍 | - |
dc.contributor.AlternativeAuthor | 강선웅 | - |
dc.contributor.AlternativeAuthor | 김주미 | - |
dc.contributor.AlternativeAuthor | 임재민 | - |
dc.contributor.AlternativeAuthor | 김효수 | - |
dc.contributor.AlternativeAuthor | 김병수 | - |
dc.contributor.AlternativeAuthor | 정형민 | - |
dc.identifier.doi | 10.1161/CIRCULATIONAHA.106.687038 | - |
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