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A teratoproteomics analysis: heat shock protein 70 is upregulated in mouse forelimb bud by methoxyacetic acid treatment

Cited 4 time in Web of Science Cited 5 time in Scopus
Authors
Ruyani, Aceng; Sudarwati, Sri; Sutasurya, Lien A.; Sumarsono, Sony H.; Kim, Dong-Jo; Chung, Jun Ho
Issue Date
2005-06-17
Publisher
Wiley-Blackwell
Citation
Birth Defects Res A Clin Mol Teratol. 2005 Jul;73(7):517-21.
Keywords
Abnormalities, Drug-InducedAcetic Acids/*toxicityAmino Acid SequenceAnimalsBlotting, WesternElectrophoresis, Gel, Two-DimensionalFemaleForelimbHSP70 Heat-Shock Proteins/*metabolismLimb Deformities, Congenital/*chemically inducedMaleMiceMolecular Sequence DataPregnancySpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*Pregnancy, AnimalTeratogens/*pharmacologyUp-Regulation
Abstract
BACKGROUND: Methoxyacetic acid (MAA) causes fetal limb abnormalities when the substance is administrated on gestation day (GD) 11 in mice. Limb abnormalities are caused mainly by extensive cell death in the mesoderm of the limb plate. This investigation focused on identifying a protein that is linked with mouse limb teratogenicity. METHODS: A single dose of MAA at 10 mmol/kg body weight was administered by gavage on GD 11; controls were administered vehicle only. Dams were killed by cervical dislocation 4 hr after treatment and forelimb buds were isolated from both the control and treated embryos. Proteins in forelimb buds GD 11 + 4 hr were precipitated out using 40-60% ammonium sulfate and were then analyzed by 2D SDS-PAGE. Excised protein spots were identified by mass spectrometry and amino acid internal sequence analysis. Identified protein was further confirmed by Western blotting. RESULTS: Two-dimensional gel analysis indicated that 1 protein spot of 81.7 kDa/pI 7.3 was overexpressed, and the protein matched heat shock protein 70 (HSP70; accession no. P08109, SwissProt). CONCLUSIONS: The results suggest that MAA, when administered to pregnant mice, upregulates HSP70 in the forelimb buds.
ISSN
1542-0752 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15959878

https://hdl.handle.net/10371/28350
DOI
https://doi.org/10.1002/bdra.20146
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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