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The identification of a Clonorchis sinensis gene encoding an antigenic egg protein

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dc.contributor.authorLee, Mejeong-
dc.contributor.authorChung, Young-Bae-
dc.contributor.authorLee, Suk-Keun-
dc.contributor.authorChung, Byung-Suk-
dc.contributor.authorLi, Shunyu-
dc.contributor.authorChoi, Min-Ho-
dc.contributor.authorHong, Sung-Tae-
dc.date.accessioned2010-01-07T06:48:00Z-
dc.date.available2010-01-07T06:48:00Z-
dc.date.issued2004-12-24-
dc.identifier.citationParasitol Res. 2005 Feb;95(3):224-6. Epub 2004 Dec 23.en
dc.identifier.issn0932-0113 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15616856-
dc.identifier.urihttp://hdl.handle.net/10371/28484-
dc.description.abstractThe cDNA library of Clonorchis sinensis was screened for genes encoding antigenic proteins by using sera from clonorchiasis patients. A gene of 888 bp encoding a 28-kDa protein (Cs28) was cloned and found to contain a high percentage of glycine (20%), tyrosine (11%), and lysine (11%). The amino acid sequence of Cs28 showed 60% homology with the vitelline B precursor protein of Opisthorchis viverrini and of 33% homology with the vitelline B1 and B2 proteins of Fasciola hepatica. A strong positive reaction was observed in the intrauterine eggs of adult C. sinensis by immunohistochemical analysis using specific immune sera against recombinant Cs28 protein (rCs28). By immunoblot analysis, rCs28 displayed an antigenic reaction with 73% of the serum samples from 115 cases of clonorchiasis. In addition, it cross-reacted with the sera of 77.5% of 40 opisthorchiasis cases, 90% of 20 schistosomiasis cases, and 50% of 10 paragonimiasis cases. However, no cross-reactions were observed with the sera of sparganosis or cysticercosis patients. In conclusion, the Cs28 protein was identified as an egg protein of C. sinensis and as an antigen common to the trematode species examined.en
dc.language.isoen-
dc.publisherSpringer Internationalen
dc.subjectAmino Acid Sequenceen
dc.subjectAnimalsen
dc.subjectAntibodies, Helminth/immunologyen
dc.subjectAntigens, Helminth/chemistry/*genetics/*immunologyen
dc.subjectCloning, Molecularen
dc.subjectClonorchiasis/diagnosis/parasitologyen
dc.subjectClonorchis sinensis/genetics/*growth & development/*immunology/isolation &en
dc.subjectpurificationen
dc.subjectEgg Proteins/chemistry/genetics/immunologyen
dc.subjectHelminth Proteins/chemistry/*genetics/immunologyen
dc.subjectHumansen
dc.subjectImmune Sera/*immunologyen
dc.subjectImmunohistochemistryen
dc.subjectMiceen
dc.subjectMice, Inbred BALB Cen
dc.subjectMolecular Sequence Dataen
dc.subjectRecombinant Proteins/immunologyen
dc.subjectSequence Analysis, DNAen
dc.titleThe identification of a Clonorchis sinensis gene encoding an antigenic egg proteinen
dc.typeArticleen
dc.contributor.AlternativeAuthor이미정-
dc.contributor.AlternativeAuthor정영배-
dc.contributor.AlternativeAuthor이석근-
dc.contributor.AlternativeAuthor정병석-
dc.contributor.AlternativeAuthor최민호-
dc.contributor.AlternativeAuthor홍성태-
dc.identifier.doi10.1007/s00436-004-1277-3-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Parasitology and Tropical Medicine (기생충학전공)Journal Papers (저널논문_기생충학전공)
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