Browse

Hydrogen peroxide increases human leukocyte adhesion to porcine aortic endothelial cells via NFkappaB-dependent up-regulation of VCAM-1

Cited 25 time in Web of Science Cited 27 time in Scopus
Authors
Lee, Sukmook; Chung, Junho; Ha, In Su; Yi, Kyesook; Lee, Ji Eun; Kang, Hee Gyung; Choi, Inho; Oh, Kook-Hwan; Kim, Jae Young; Surh, Charles D; Ahn, Curie
Issue Date
2007-11-06
Publisher
Oxford University Press
Citation
Int Immunol. 2007 Dec;19(12):1349-59. Epub 2007 Oct 29.
Keywords
AnimalsAorta/cytologyCell AdhesionCell LineEndothelial Cells/drug effects/*metabolismHumansHydrogen Peroxide/*metabolism/pharmacologyLeukocytes/drug effects/*metabolismNF-kappa B/*metabolismSwineTumor Necrosis Factor-alpha/metabolismUmbilical VeinsVascular Cell Adhesion Molecule-1/*metabolism
Abstract
Although a severe shortage of organs in transplantation can be overcome by using xenotransplantation of porcine donor organs, profound immune rejection to xenogeneic antigens remains a main obstacle. To elucidate the role of hydrogen peroxide (H(2)O(2)) on xenogeneic immune responses, we investigated its effects on porcine aortic endothelial cells (PAECs). We found that H(2)O(2) can specifically induce vascular cell adhesion molecule-1 (VCAM-1) expression on PAECs, but little on human umbilical vein endothelial cells (HUVECs) and aortic endothelial cells (HAECs). Furthermore, we further confirmed that H(2)O(2) induces activation of NFkappaB in PAECs, but not in HAECs. Interestingly, cell adhesion assay showed that U937, human promonocytic leukocyte, can adhere to PAECs in an H(2)O(2)-dependent manner and by using a neutralizing assay with anti-VCAM-1-specific antibodies, we also found that the interaction is mediated primarily by VCAM-1. Finally, we also demonstrated that up-regulation of VCAM-1 expression on PAECs by reactive oxygen species-producing HL-60, human leukemic neutrophil cells, could be significantly diminished by over-expressing an H(2)O(2)-removing catalase. In summary, our results suggest that NFkappaB-dependent porcine VCAM-1 expression by H(2)O(2) may promote interaction of human leukocyte to PAECs, and thus may play an important role on inducing xenogeneic immune responses.
ISSN
0953-8178 (Print)
Language
English
URI
http://intimm.oxfordjournals.org/cgi/content/abstract/19/12/1349

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17977815

http://hdl.handle.net/10371/28933
DOI
https://doi.org/10.1093/intimm/dxm104
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse