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Co-culture of human CD34+ cells with mesenchymal stem cells increases the survival of CD34+ cells against the 5-aza-deoxycytidine- or trichostatin A-induced cell death

Cited 19 time in Web of Science Cited 21 time in Scopus
Authors
Koh, Sang Hyeok; Choi, Hyoung Soo; Park, Eun Sil; Kang, Hyoung Jin; Ahn, Hyo Seop; Shin, Hee Young
Issue Date
2005-03-09
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2005 Apr 15;329(3):1039-45.
Keywords
Antigens, CD34/*metabolismApoptosis/drug effects/*physiologyAzacitidine/*analogs & derivatives/*pharmacologyCell Differentiation/drug effectsCell Proliferation/drug effectsCell Size/drug effectsCell Survival/drug effects/*physiologyCells, CulturedCoculture Techniques/methodsHematopoietic Stem Cells/drug effects/*physiologyHumansHydroxamic Acids/*pharmacologyMesenchymal Stem Cells/cytology/drug effects/*physiologyTissue Engineering/methods
Abstract
It has been suggested that epigenetic regulation plays an important role in maintaining the stemness and lineage differentiation of hematopoietic stem cells (HSCs), 5-aza-deoxycytidine (aza-D) and Trichostatin A (TSA) being candidate additives for HSC ex vivo expansion. Although they have potent activity to maintain the stemness, they can also cause serious cell death. This study examined the effects of mesenchymal stem cells (MSCs) on the maintenance of CD34+ cells driven by aza-D and TSA in culture with the combined cytokines of thrombopoietin, flt-3 ligand, stem cell factor, interleukin-3, and interleukin-6. In cultures without MSCs, although aza-D and TSA retained the CD34 frequency 4 to 8 times more than in the cytokines alone, a large portion of cells underwent apoptotic cell death. Consequently, CD34+ cell expansion could not be achieved in any condition without MSCs. In cultures with MSCs, the total cell number was higher in aza-D or TSA than in any conditions in the cultures without MSCs. The CD34 frequency was also similar to the level in the cultures in aza-D or TSA without the MSCs. These results suggest that a co-culture of CD34+ cells with the MSCs might not simply deliver the proliferation signals but also stemness and survival signals, and overlap the action of epigenetic regulators.
ISSN
0006-291X (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4FHJF00-7&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=d87446b9aeedb4dc3c27c396a8692eb1

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15752760

https://hdl.handle.net/10371/29087
DOI
https://doi.org/10.1016/j.bbrc.2005.02.077
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College of Medicine/School of Medicine (의과대학/대학원)Pediatrics (소아과학전공)Journal Papers (저널논문_소아과학전공)
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