S-Space College of Medicine/School of Medicine (의과대학/대학원) Anesthesiology and Pain Medicine (마취통증의학전공) Journal Papers (저널논문_마취통증의학전공)
The effect of thalidomide on spinal cord ischemia/reperfusion injury in a rabbit model
- Lee, C. J.; Kim, K. W.; Lee, H. M.; Nahm, F. S.; Lim, Y. J.; Park, J. H.; Kim, C. S.
- Issue Date
- Nature Publishing Group
- Spinal Cord. 2007 Feb;45(2):149-57. Epub 2006 Mar 28.
- Animals; Blood Pressure/drug effects/physiology; Blotting, Western/methods; Cell Count; Disease Models, Animal; Immunosuppressive Agents/*therapeutic use; Ischemia/*complications; Male; Motor Neurons/pathology; Neurologic Examination/methods; Rabbits; Reperfusion Injury/*complications; Spinal Cord Injuries/*drug therapy/etiology/pathology; Thalidomide/*therapeutic use; Time Factors; Tumor Necrosis Factor-alpha/metabolism
- STUDY DESIGN: Randomized study. OBJECTIVES: To evaluate the effects of thalidomide on spinal cord ischemia/reperfusion injury via reduced TNF-alpha production. SETTING: Animal experimental laboratory, Clinical Research Institute of Seoul National University Hospital, Seoul, Korea. METHODS: Spinal cord ischemia was induced in rabbits by occluding the infrarenal aorta. Rabbits in group N did not undergo ischemic insult, but rabbits in groups C (the untreated group), THA, and THB underwent ischemic insult for 15 min. The THA and THB groups received thalidomide (20 mg/kg) intraperitoneally (i.p.) before ischemia, but only the THB group received thalidomide (i.p., 20 mg/kg) after 24 and 48 h of reperfusion. After evaluating neurologic functions at 1.5 h, 3, and 5 days of reperfusion, rabbits were killed for histopathologic examination and Western blot analysis of TNF-alpha. RESULTS: The THA and THB groups showed significantly less neurologic dysfunction than the C group at 1.5 h, 3, and 5 days of reperfusion. The number of normal spinal motor neurons in ventral gray matter was higher in THA and THB than in C, but no difference was observed between THA and THB. Western blot analysis showed a significantly higher level of TNF-alpha in C than in THA and THB at 1.5 h of reperfusion, but no difference was observed between C, THA, or THB at 3 or 5 days of reperfusion. CONCLUSION: Thalidomide treatment before ischemic insult reduces early phase ischemia/reperfusion injury of the spinal cord in rabbits.
- 1362-4393 (Print)
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