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Morphological and biochemical changes induced by arsenic trioxide in neuroblastoma cell lines
DC Field | Value | Language |
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dc.contributor.author | Ryu, Kyung-Ha | - |
dc.contributor.author | Woo, So-Youn | - |
dc.contributor.author | Lee, Mi-Young | - |
dc.contributor.author | Jung, Yun-Jae | - |
dc.contributor.author | Yoo, Eun-Sun | - |
dc.contributor.author | Seoh, Ju-Young | - |
dc.contributor.author | Kie, Jeong-Hae | - |
dc.contributor.author | Shin, Hee-Young | - |
dc.contributor.author | Ahn, Hyo-Seop | - |
dc.date.accessioned | 2010-01-11T07:35:49Z | - |
dc.date.available | 2010-01-11T07:35:49Z | - |
dc.date.issued | 2005-09-17 | - |
dc.identifier.citation | Pediatr Hematol Oncol. 2005 Oct-Nov;22(7):609-21. | en |
dc.identifier.issn | 0888-0018 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16166054 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29395 | - |
dc.description.abstract | Arsenic trioxide has recently been shown to inhibit growth and induce apoptosis in a variety of hematologic malignancies, but very little is known about its effects on solid tumors and especially on neuroblastoma cells that have self-differentiating characteristics. To demonstrate the growth inhibition induced in neuroblastoma cells (the SH-SY5Y and SK-N-AS cell line) and acute promyelocytic leukemia cells (HL-60) by arsenic trioxide (As2O3), the viable cell numbers were counted after trypan blue staining. Apoptosis was assessed by the cell morphology, by flow cytometry with annexin-V staining, and by Western blot analysis for the apoptosis-related proteins (bcl-2 and PARP). To decide the dose for the clinical application of As2O3, normal peripheral blood lymphocytes were also examined. The growth and survival of the SH-SY5Y and SK-N-AS cells were markedly inhibited by As2O3 treatment at a 3 microM concentration before the changes of the normal lymphocytes were observed. The apoptotic cells showed a shrunken cell nucleus, and an increase in the number and balloon-like swelling of the mitochondria at 72 h after the As2O3 was added. Apoptosis of the annexin-V-positive cell proportion in the neuroblastoma cell lines was increased with increasing the exposure time and the concentration of As2O3, just like the HL-60 cells. Bcl-2 downregulation and PARP degradation were also noted all the cell lines, but these changes were not statistically significant among the 3 cell lines. Taken together, these results indicate that As2O3 is an excellent candidate as a therapeutic agent for the treatment of neuroblastoma. | en |
dc.language.iso | en | en |
dc.publisher | Taylor & Francis | en |
dc.subject | Annexin A5/biosynthesis | en |
dc.subject | Antineoplastic Agents/*pharmacology/therapeutic use | en |
dc.subject | Apoptosis/*drug effects | en |
dc.subject | Arsenicals/*pharmacology/therapeutic use | en |
dc.subject | Cell Survival/drug effects | en |
dc.subject | Collagen Type XI/biosynthesis | en |
dc.subject | Dose-Response Relationship, Drug | en |
dc.subject | Down-Regulation/drug effects | en |
dc.subject | Gene Expression Regulation, Neoplastic/drug effects | en |
dc.subject | HL-60 Cells | en |
dc.subject | Humans | en |
dc.subject | Lymphocytes/metabolism/ultrastructure | en |
dc.subject | Neuroblastoma/drug therapy/*metabolism/ultrastructure | en |
dc.subject | Oxides/*pharmacology/therapeutic use | en |
dc.subject | Proto-Oncogene Proteins c-bcl-2/biosynthesis | en |
dc.title | Morphological and biochemical changes induced by arsenic trioxide in neuroblastoma cell lines | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 유경하 | - |
dc.contributor.AlternativeAuthor | 유소연 | - |
dc.contributor.AlternativeAuthor | 이미영 | - |
dc.contributor.AlternativeAuthor | 정연재 | - |
dc.contributor.AlternativeAuthor | 유은선 | - |
dc.contributor.AlternativeAuthor | 서주영 | - |
dc.contributor.AlternativeAuthor | 기정해 | - |
dc.contributor.AlternativeAuthor | 신희영 | - |
dc.contributor.AlternativeAuthor | 안효섭 | - |
dc.identifier.doi | 10.1080/08880010500198897 | - |
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