S-Space College of Medicine/School of Medicine (의과대학/대학원) Program in Cancer Biology (협동과정-종양생물학전공) Journal Papers (저널논문_협동과정-종양생물학전공)
Role of complement regulatory proteins in the survival of murine allo-transplanted Sertoli cells
- Lee, Hak-Mo; Oh, Byoung Chol; Lim, Dong-Pyo; Lee, Dong-Sup; Cho, Jaejin; Lee, Gene; Lee, Jeong Ryul
- Issue Date
- Korean Academy of Medical Science
- J Korean Med Sci. 2007 Apr;22(2):277-82.
- Animals; Cell Survival; Cells, Cultured; Clusterin/*immunology; Complement System Proteins/*immunology; Fas Ligand Protein/*immunology; Female; Graft Survival/*immunology; Male; Mice; Mice, Inbred C57BL; Sertoli Cells/*immunology/*transplantation; Transforming Growth Factor beta1/*immunology; Transplantation, Homologous/immunology
- Sertoli cells (SC) are known to contain immunoprotective properties, which allow them to survive as allografts without the use of immunosuppressive drugs. Experiments were designed to determine which factors are related to prolonged survival of allogeneic SC. Balb/c derived Sertoli (TM4) and colon cancer (CT-26) cell lines were implanted beneath the kidney capsule of non-immunosuppressed C57BL/6 mice and compared their survival as allografts. Compared to TM4 graft, which survived more than 7 days after transplantation, CT-26 showed massive infiltration of polymorphonuclear cells, necrosis and enlargement of draining lymph nodes. Cultured cell lines showed no differences in their expression patterns of FasL, TGF beta1, clusterin and two complement regulatory proteins (CRP, i.e., membrane cofactor protein, MCP; decay accelerating factor, DAF), but protectin (CD59), another member of CRP was expressed only on TM4. These results suggest that CD59 and unknown factors may contribute to the prolonged survival of SC in non-immunoprivileged sites.
- 1011-8934 (Print)