Browse

Erythroid cell-specific alpha-globin gene regulation by the CP2 transcription factor family

Cited 24 time in Web of Science Cited 23 time in Scopus
Authors
Kang, Ho Chul; Chae, Ji Hyung; Lee, Yeon Ho; Park, Mi-Ae; Shin, June Ho; Kim, Sung-Hyun; Ye, Sang-Kyu; Cho, Yoon Shin; Fiering, Steven; Kim, Chul Geun
Issue Date
2005-07-01
Publisher
American Society for Microbiology
Citation
Mol. Cell Biol. 25:6005-6020
Keywords
Alternative SplicingAnimalsCell DifferentiationCells, CulturedDNA-Binding Proteins/genetics/*metabolismDimerizationErythroid Cells/*metabolismGene ExpressionGlobins/*geneticsHumansMicePromoter Regions, Genetic/geneticsProtein Inhibitors of Activated STATProtein Isoforms/genetics/metabolismProteins/*metabolismRNA-Binding Proteins/genetics/*metabolismTranscription Factors/genetics/*metabolism*Transcriptional Activation
Abstract
We previously demonstrated that ubiquitously expressed CP2c exerts potent erythroid-specific transactivation of alpha-globin through an unknown mechanism. This mechanism is reported here to involve specific CP2 splice variants and protein inhibitor of activated STAT1 (PIAS1). We identify a novel murine splice isoform of CP2, CP2b, which is identical to CP2a except that it has an additional 36 amino acids encoded by an extra exon. CP2b has an erythroid cell-specific transcriptional activation domain, which requires the extra exon and can form heteromeric complexes with other CP2 isoforms, but lacks the DNA binding activity found in CP2a and CP2c. Transcriptional activation of alpha-globin occurred following dimerization between CP2b and CP2c in erythroid K562 and MEL cells, but this dimerization did not activate the alpha-globin promoter in nonerythroid 293T cells, indicating that an additional erythroid factor is missing in 293T cells. PIAS1 was confirmed as a CP2 binding protein by the yeast two-hybrid screen, and expression of CP2b, CP2c, and PIAS1 in 293T cell induced alpha-globin promoter activation. These results show that ubiquitously expressed CP2b exerts potent erythroid cell-specific alpha-globin gene expression by complexing with CP2c and PIAS1.
ISSN
0270-7306 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15988015

http://hdl.handle.net/10371/29504
DOI
https://doi.org/10.1128/MCB.25.14.6005-6020.2005
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pharmacology (약리학전공)Journal Papers (저널논문_약리학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse