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Synergistic neovascularization by mixed transplantation of early endothelial progenitor cells and late outgrowth endothelial cells: the role of angiogenic cytokines and matrix metalloproteinases

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dc.contributor.authorYoon, Chang-Hwan-
dc.contributor.authorHur, Jin-
dc.contributor.authorPark, Kyung-Woo-
dc.contributor.authorKim, Ji-Hyun-
dc.contributor.authorLee, Choon-Soo-
dc.contributor.authorOh, Il-Young-
dc.contributor.authorKim, Tae-Youn-
dc.contributor.authorCho, Hyun-Jai-
dc.contributor.authorKang, Hyun-Jae-
dc.contributor.authorChae, In-Ho-
dc.contributor.authorYang, Han-Kwang-
dc.contributor.authorOh, Byung-Hee-
dc.contributor.authorPark, Young-Bae-
dc.contributor.authorKim, Hyo-Soo-
dc.date.accessioned2010-01-12T01:47:30Z-
dc.date.available2010-01-12T01:47:30Z-
dc.date.issued2005-09-08-
dc.identifier.citationCirculation. 2005 Sep 13;112(11):1618-27. Epub 2005 Sep 6.en
dc.identifier.issn1524-4539 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16145003-
dc.identifier.urihttps://hdl.handle.net/10371/29523-
dc.description.abstractBACKGROUND: Two types of cells are cultured from the human peripheral blood, early endothelial progenitor cells (EPCs) and outgrowth endothelial cells (OECs), as previously reported. Here, we further characterize these cells, especially with respect to their different origins and functions both in vitro and in vivo. We also investigated whether the combination of these different cell types shows synergism during neovascularization. METHODS AND RESULTS: Early EPCs were heterogeneously made up of both CD14+ monocyte-derived cells, which secrete cytokines, and CD14(-)-derived cells, which contain high levels of (CD34+)KDR+ cells. OECs were cultured almost exclusively from CD14- cells, not CD14+ cells, and were distinct from mature endothelial cells in terms of proliferation potential, KDR+ expression level, and telomerase activity. A portion of cells from CD14- cells and early EPCs produced rapidly proliferating, capillary-forming cells in both the Matrigel plug and the ischemic hind limb similar to OECs. Early EPCs and OECs expressed receptors for vascular endothelial growth factor and interleukin-8, cytokines secreted by early EPCs. There was a differential increase in matrix metalloproteinases (MMPs): MMP-9 in early EPCs and MMP-2 in OECs. In vitro, the angiogenic capability of the 2 cell types was augmented by mutual interaction through cytokines and MMPs. Injection of a mixture of the 2 cells resulted in superior neovascularization in vivo to any single-cell-type transplantation. CONCLUSIONS: Distinct origins of the different types of EPCs exist that have different functions in neovascularization. Mixed transplantation of these cells results in synergistic neovascularization through cytokines and MMPs.en
dc.language.isoen-
dc.publisherAmerican Heart Associationen
dc.subjectAnimalsen
dc.subjectAutocrine Communicationen
dc.subjectBlood Cells/cytologyen
dc.subjectCell Agingen
dc.subjectCell Lineageen
dc.subjectCells, Cultureden
dc.subjectCytokines/*metabolismen
dc.subjectEndothelial Cells/metabolism/*transplantationen
dc.subjectFemaleen
dc.subjectHindlimb/*blood supplyen
dc.subjectHumansen
dc.subjectInterleukin-8/metabolismen
dc.subjectIschemia/physiopathology/*surgeryen
dc.subjectMatrix Metalloproteinases/*metabolismen
dc.subjectMiceen
dc.subjectMice, Nudeen
dc.subject*Neovascularization, Physiologicen
dc.subjectParacrine Communicationen
dc.subject*Stem Cell Transplantationen
dc.subjectStem Cells/metabolismen
dc.subjectVascular Endothelial Growth Factor A/metabolismen
dc.titleSynergistic neovascularization by mixed transplantation of early endothelial progenitor cells and late outgrowth endothelial cells: the role of angiogenic cytokines and matrix metalloproteinasesen
dc.typeArticleen
dc.contributor.AlternativeAuthor윤창환-
dc.contributor.AlternativeAuthor허진-
dc.contributor.AlternativeAuthor박경우-
dc.contributor.AlternativeAuthor김지현-
dc.contributor.AlternativeAuthor이춘수-
dc.contributor.AlternativeAuthor오일영-
dc.contributor.AlternativeAuthor김태윤-
dc.contributor.AlternativeAuthor조현재-
dc.contributor.AlternativeAuthor강현재-
dc.contributor.AlternativeAuthor채인호-
dc.contributor.AlternativeAuthor양한광-
dc.contributor.AlternativeAuthor오병희-
dc.contributor.AlternativeAuthor박영배-
dc.contributor.AlternativeAuthor김효수-
dc.identifier.doi10.1161/CIRCULATIONAHA.104.503433-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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