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Direct application of AvaII PCR restriction fragment length polymorphism analysis (AvaII PRA) targeting 644 bp heat shock protein 65 (hsp65) gene to sputum samples

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dc.contributor.authorMun, Ho-Suk-
dc.contributor.authorKim, Hyun-Ju-
dc.contributor.authorOh, Eun-Ju-
dc.contributor.authorKim, Hong-
dc.contributor.authorPark, Young-Gil-
dc.contributor.authorBai, Gil-Han-
dc.contributor.authorDo, Junghwan-
dc.contributor.authorCha, Chang-Yong-
dc.contributor.authorKook, Yoon-Hoh-
dc.contributor.authorKim, Bum-Joon-
dc.date.accessioned2010-01-12T01:54:37Z-
dc.date.available2010-01-12T01:54:37Z-
dc.date.issued2007-01-24-
dc.identifier.citationMicrobiol Immunol. 2007;51(1):105-10.en
dc.identifier.issn0385-5600 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17237605-
dc.identifier.urihttps://hdl.handle.net/10371/29539-
dc.description.abstractTo evaluate the usefulness of the AvaII PRA method targeting 644-bp hsp65 gene for the direct detection of pathogenic mycobacteria from clinical specimens, we applied this method to 40 sputum samples and compared the results to those obtained by IS 6110 PCR. Although this method showed a sensitivity slightly lower than IS 6110 PCR (97.5% vs. 100%), it detected infections of M. avium complex (MAC) in two patients, which was not possible by IS 6110 PCR. We conclude that AvaII PRA is a highly effective method for directly detecting pathogenic mycobacteria in primary clinical specimens.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectBacterial Proteins/*geneticsen
dc.subjectBacteriological Techniquesen
dc.subjectChaperonins/*geneticsen
dc.subject*DNA Fingerprintingen
dc.subjectDNA, Bacterial/*genetics/metabolismen
dc.subjectDeoxyribonucleases, Type II Site-Specific/metabolismen
dc.subjectHumansen
dc.subjectMycobacterium/genetics/*isolation & purificationen
dc.subjectPolymerase Chain Reactionen
dc.subject*Polymorphism, Restriction Fragment Lengthen
dc.subjectSensitivity and Specificityen
dc.subjectSputum/*microbiologyen
dc.titleDirect application of AvaII PCR restriction fragment length polymorphism analysis (AvaII PRA) targeting 644 bp heat shock protein 65 (hsp65) gene to sputum samplesen
dc.typeArticleen
dc.contributor.AlternativeAuthor문호숙-
dc.contributor.AlternativeAuthor김현주-
dc.contributor.AlternativeAuthor오은경-
dc.contributor.AlternativeAuthor김홍-
dc.contributor.AlternativeAuthor박영길-
dc.contributor.AlternativeAuthor배길한-
dc.contributor.AlternativeAuthor도중환-
dc.contributor.AlternativeAuthor차창용-
dc.contributor.AlternativeAuthor국윤호-
dc.contributor.AlternativeAuthor김범준-
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