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Identification, evolution, and regulation of expression of Guinea pig trappin with an unusually long transglutaminase substrate domain

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dc.contributor.authorFurutani, Yutaka-
dc.contributor.authorKato, Akira-
dc.contributor.authorFibriani, Azzania-
dc.contributor.authorHirata, Taku-
dc.contributor.authorKawai, Ryoji-
dc.contributor.authorJeon, Ju-Hong-
dc.contributor.authorFujii, Yasuhisa-
dc.contributor.authorKim, In-Gyu-
dc.contributor.authorKojima, Soichi-
dc.contributor.authorHirose, Shigehisa-
dc.date.accessioned2010-01-12T04:32:07Z-
dc.date.available2010-01-12T04:32:07Z-
dc.date.issued2005-03-22-
dc.identifier.citationJ Biol Chem. 2005 May 27;280(21):20204-15. Epub 2005 Mar 18.en
dc.identifier.issn0021-9258 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15778505-
dc.identifier.urihttps://hdl.handle.net/10371/29647-
dc.description.abstractTrappins are found in human, bovine, hippopotamus, and members of the pig family, but not in rat and mouse. To clarify the evolution of the trappin genes and the functional significance of their products, we isolated the trappin gene in guinea pig, a species belonging to a rodent family distinct from rat and mouse. Guinea pig trappin was confirmed to encode the same domain structure as trappin, consisting of a signal sequence, an extra large transglutaminase substrate domain, and a whey acidic protein motif. Northern blot analysis and in situ hybridization histochemistry as well as immunohistochemistry demonstrated that guinea pig trappin is expressed solely in the secretory epithelium of the seminal vesicle and that its expression is androgen-dependent. We confirmed that guinea pig trappin is cross-linked by prostate transglutaminase and that the whey acidic protein motif derived from guinea pig trappin has an inhibitory activity against leukocyte elastase. Genome sequence analysis showed that guinea pig trappin belongs to the family of REST (rapidly evolving seminal vesicle transcribed) genes.en
dc.language.isoenen
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen
dc.subjectAmino Acid Sequenceen
dc.subjectAndrogens/pharmacologyen
dc.subjectAnimalsen
dc.subjectBinding Sitesen
dc.subjectBlotting, Westernen
dc.subjectCalcium/pharmacologyen
dc.subjectCattleen
dc.subjectCross-Linking Reagentsen
dc.subjectDNA, Complementary/chemistryen
dc.subjectGuinea Pigsen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectLeukocyte Elastase/antagonists & inhibitorsen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMilk Proteins/chemistry/metabolismen
dc.subjectMolecular Sequence Dataen
dc.subjectProstate/enzymologyen
dc.subjectProteins/*analysis/chemistry/*geneticsen
dc.subjectProteinase Inhibitory Proteins, Secretoryen
dc.subjectRatsen
dc.subjectSeminal Vesicles/chemistryen
dc.subjectSequence Alignmenten
dc.subjectSwineen
dc.subjectTissue Distributionen
dc.subjectTransglutaminases/*metabolismen
dc.subjectEvolution, Molecular-
dc.subjectGene Expression Regulation/drug effects-
dc.titleIdentification, evolution, and regulation of expression of Guinea pig trappin with an unusually long transglutaminase substrate domainen
dc.typeArticleen
dc.contributor.AlternativeAuthor김인규-
dc.identifier.doi10.1074/jbc.M501678200-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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