Browse

Cabin1 represses MEF2 transcriptional activity by association with a methyltransferase, SUV39H1

DC Field Value Language
dc.contributor.authorJang, Hyonchol-
dc.contributor.authorChoi, Doo-Eun-
dc.contributor.authorKim, Hyungsoo-
dc.contributor.authorCho, Eun-Jung-
dc.contributor.authorYoun, Hong-Duk-
dc.date.accessioned2010-01-12T04:34:17Z-
dc.date.available2010-01-12T04:34:17Z-
dc.date.issued2006-12-19-
dc.identifier.citationJ Biol Chem. 2007 Apr 13;282(15):11172-9. Epub 2006 Dec 17.en
dc.identifier.issn0021-9258 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17172641-
dc.identifier.urihttp://hdl.handle.net/10371/29651-
dc.description.abstractMyocyte enhancer factor 2 (MEF2) plays pivotal roles in various biological processes, and its transcriptional activity is regulated by histone acetylation/deacetylation enzymes in a calcium-dependent fashion. A calcineurin-binding protein 1 (Cabin1) has been shown to participate in repression of MEF2 by recruiting mSin3 and its associated histone deacetylases. Here, we report that histone methylation also takes a part in Cabin1-mediated repression of MEF2. Immunoprecipitate of Cabin1 complex can methylate histone H3 by association with SUV39H1. SUV39H1 increased Cabin1-mediated repression of MEF2 transcriptional activity in MEF2-targeting promoters. The SUV39H1 was revealed to bind to the 501-900-amino acid region of Cabin1, which was distinct from its histone deacetylase-recruiting domain. In addition, the Gal4-Cabin1-(501-900) alone repressed a constitutively active Gal4-tk-promoter, indicating that Cabin1 recruits SUV39H1 and represses transcriptional activity. Finally, both SUV39H1 and Cabin1 were shown to bind on the MEF2 target promoter in a calcium-dependent manner. Thus, Cabin1 recruits chromatin-modifying enzymes, both histone deacetylases and a histone methyltransferase, to repress MEF2 transcriptional activity.en
dc.language.isoenen
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen
dc.subjectAmino Acids/genetics/metabolismen
dc.subjectCalcium/metabolismen
dc.subjectCell Lineen
dc.subjectHistone-Lysine N-Methyltransferase/genetics/*metabolismen
dc.subjectHumansen
dc.subjectMyogenic Regulatory Factors/*genetics/*metabolismen
dc.subjectPhosphoproteins/genetics/*metabolismen
dc.subjectPromoter Regions, Genetic/geneticsen
dc.subjectProtein Bindingen
dc.subjectProtein Methyltransferasesen
dc.subjectTranscription, Genetic/*geneticsen
dc.titleCabin1 represses MEF2 transcriptional activity by association with a methyltransferase, SUV39H1en
dc.typeArticleen
dc.contributor.AlternativeAuthor장현철-
dc.contributor.AlternativeAuthor김형수-
dc.contributor.AlternativeAuthor조은정-
dc.contributor.AlternativeAuthor윤홍덕-
dc.identifier.doi10.1074/jbc.M611199200-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Program in Cancer Biology (협동과정-종양생물학전공)Journal Papers (저널논문_협동과정-종양생물학전공)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse