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The endogenous ratio of Smad2 and Smad3 influences the cytostatic function of Smad3
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sang Gyun | - |
dc.contributor.author | Kim, Hyun-Ah | - |
dc.contributor.author | Jong, Hyun-Soon | - |
dc.contributor.author | Park, Jung-Hyun | - |
dc.contributor.author | Kim, Noe Kyeong | - |
dc.contributor.author | Hong, Seung Hwan | - |
dc.contributor.author | Kim, Tae-You | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.date.accessioned | 2010-01-12T04:57:24Z | - |
dc.date.available | 2010-01-12T04:57:24Z | - |
dc.date.issued | 2005-08-12 | - |
dc.identifier.citation | Mol Biol Cell. 2005 Oct;16(10):4672-83. Epub 2005 Aug 10. | en |
dc.identifier.issn | 1059-1524 (Print) | - |
dc.identifier.issn | http://dx.doi.org/10.1091/mbc.E05-01-0054 | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16093355 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29670 | - |
dc.description.abstract | Although Smad2 and Smad3, critical transcriptional mediators of transforming growth factor-beta (TGF-beta) signaling, are supposed to play a role in the TGF-beta cytostatic program, it remains unclear whether TGF-beta delivers cytostatic signals through both Smads equally or through either differentially. Here, we report that TGF-beta cytostatic signals rely on a Smad3-, but not a Smad2-, dependent pathway and that the intensity of TGF-beta cytostatic signals can be modulated by changing the endogenous ratio of Smad3 to Smad2. Depleting endogenous Smad3 by RNA interference sufficiently interfered with TGF-beta cytostatic actions in various TGF-beta-sensitive cell lines, whereas raising the relative endogenous ratio of Smad3 to Smad2, by depleting Smad2, markedly enhanced TGF-beta cytostatic response. Consistently, Smad3 activation and its transcriptional activity upon TGF-beta stimulation were facilitated in Smad2-depleted cells relative to controls. Most significantly, a single event of increasing this ratio by Smad2 depletion was sufficient to restore TGF-beta cytostatic action in cells resistant to TGF-beta. These findings suggest a new important determinant of sensitivity to TGF-beta cytostatic signaling. | en |
dc.language.iso | en | en |
dc.publisher | American Society for Cell Biology | en |
dc.subject | Animals | en |
dc.subject | Cell Cycle | en |
dc.subject | Cell Line | en |
dc.subject | Cell Proliferation | en |
dc.subject | Cytoplasm/physiology | en |
dc.subject | Epithelial Cells/physiology | en |
dc.subject | Gene Expression Regulation | en |
dc.subject | Genes, Reporter | en |
dc.subject | Humans | en |
dc.subject | Mink | en |
dc.subject | RNA, Small Interfering/genetics | en |
dc.subject | Signal Transduction | en |
dc.subject | Smad2 Protein/genetics/*physiology | en |
dc.subject | Smad3 Protein/genetics/*physiology | en |
dc.subject | Transforming Growth Factor beta/*physiology | en |
dc.title | The endogenous ratio of Smad2 and Smad3 influences the cytostatic function of Smad3 | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김상균 | - |
dc.contributor.AlternativeAuthor | 김현아 | - |
dc.contributor.AlternativeAuthor | 종현순 | - |
dc.contributor.AlternativeAuthor | 박정현 | - |
dc.contributor.AlternativeAuthor | 김노경 | - |
dc.contributor.AlternativeAuthor | 홍승환 | - |
dc.contributor.AlternativeAuthor | 김태유 | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
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