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Cyclooxygenase-2 inhibits novel ginseng metabolite-mediated apoptosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, Hyung Woo | - |
dc.contributor.author | Jong, Hyun-Soon | - |
dc.contributor.author | Kim, Tai Young | - |
dc.contributor.author | Choi, Hyun Ho | - |
dc.contributor.author | Kim, Sang Gyun | - |
dc.contributor.author | Song, Sang Hyun | - |
dc.contributor.author | Kim, Juyong | - |
dc.contributor.author | Ko, Seong-Gyu | - |
dc.contributor.author | Lee, Jung Weon | - |
dc.contributor.author | Kim, Tae-You | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.date.accessioned | 2010-01-12T05:22:38Z | - |
dc.date.available | 2010-01-12T05:22:38Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2005-03 | - |
dc.identifier.citation | Cancer Research, Vol.65 No.5, pp.1952-1960 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.other | 4031 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29689 | - |
dc.description.abstract | Recently, a novel intestinal bacterial metabolite of ginseng protopanaxadiol saponins, i.e., 20-0-(beta-D-glucopyranosyl)20(S)-protopanaxadiol (1H-901), has been reported to induce apoptosis in a variety of cancer cells. Here we show a differential effect of IH-901 on several cell types. Exposure to IH-901 for 48 hours at a supposedly subapoptotic concentration of 40 mumol/L led to both apoptotic cell death and G(1) arrest in Hep3B cells, but only resulted in G(1) arrest in MDAMB-231, Hs578T, and MKN28 cells. Additionally, the treatment of MDA-MB-231, but not of Hep3B, with IH-901 up-regulated cyclooxygenase-2 (COX-2) mRNA (2 hours) and protein (6 hours), and enhanced the production of prostaglandin E2. In MDA-MB-231 cells, IH=901 induced the sustained activation of extracellular signal-regulated kinase (ERK), whereas inhibition of mitogen-activated protein/ERK kinase blocked 1H901-mediated COX-2 induction and resulted in apoptosis, suggesting the involvement of an ERK-COX-2 pathway. Combined treatment with IH-901 and nonsteroidal anti-inflammatory drugs inhibited COX-2 enzyme and induced apoptosis in MDA-MB-231 and Hs578T cells. Adenovirus-mediated COX-2 small interfering RNAs also effectively inhibited COX-2 protein expression and enhanced IH-901-mediated apoptosis without inhibiting ERK 1/2 phosphorylation, thus providing direct evidence that COX-2 is an antiapoptotic molecule. Moreover, IH-901-mediated G(1) arrest resulted from an increase in p27(KiP1) mRNA and protein expression followed by a decrease in CDK2 kinase activity that was concurrent with the hypophosphorylation of Rb and p130. In conclusion, IH-901 induced both G(1) arrest and apoptosis, and this apoptosis could be inhibited by COX-2 induction. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Association for Cancer Research | - |
dc.title | Cyclooxygenase-2 inhibits novel ginseng metabolite-mediated apoptosis | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-04-1740 | - |
dc.citation.journaltitle | Cancer Research | - |
dc.identifier.wosid | 000227294600043 | - |
dc.identifier.scopusid | 2-s2.0-20144388887 | - |
dc.citation.endpage | 1960 | - |
dc.citation.number | 5 | - |
dc.citation.startpage | 1952 | - |
dc.citation.volume | 65 | - |
dc.identifier.sci | 000227294600043 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Sang Gyun | - |
dc.contributor.affiliatedAuthor | Lee, Jung Weon | - |
dc.contributor.affiliatedAuthor | Kim, Tae-You | - |
dc.contributor.affiliatedAuthor | Bang, Yung-Jue | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | HUMAN GASTRIC-CARCINOMA | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | CYCLE ARREST | - |
dc.subject.keywordPlus | INTESTINAL BACTERIA | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SAPONIN | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
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