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O-GlcNAc modulation at Akt1 Ser473 correlates with apoptosis of murine pancreatic β cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Jung Weon | - |
dc.contributor.author | Kang, Eun-Sil | - |
dc.contributor.author | Han, Dohyun | - |
dc.contributor.author | Park, Jungeun | - |
dc.contributor.author | Kwak, Tae Kyoung | - |
dc.contributor.author | Oh, Min-A | - |
dc.contributor.author | Lee, Sin-Ae | - |
dc.contributor.author | Choi, Suyong | - |
dc.contributor.author | Park, Zee Yong | - |
dc.contributor.author | Kim, Youngsoo | - |
dc.date.accessioned | 2009-05-15T04:25:17Z | - |
dc.date.available | 2009-05-15T04:25:17Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Exp.Cell Res. 314(2008) 2238-2248 | en |
dc.identifier.issn | 0014-4827 | - |
dc.identifier.uri | http://www.elsevier.com/locate/yexcr | - |
dc.identifier.uri | https://hdl.handle.net/10371/3239 | - |
dc.description.abstract | O-GlcNAc transferase (OGT)-mediated modification of protein Ser/Thr residues withO-GlcNAc influences protein activity, similar to the effects of phosphorylation. The anti-apoptotic Akt1 is both activated by phosphorylation and modified with O-GlcNAc. However, the nature and significance of the Akt1 O-GlcNAc modification is unknown. The relationship of O-GlcNAc modification and phosphorylation at Akt1 Ser473 was examined with respect to apoptosis of murine β-pancreatic cells. Glucosamine treatment induced apoptosis, which correlated with enhanced O-GlcNAc modification of Akt1 and concomitant reduction in Ser473 phosphorylation. Pharmacological inhibition of OGT or O-GlcNAcase revealed an inverse correlation between O-GlcNAcmodification and Ser473 phosphorylation of Akt1. MALDI-TOF/TOFmass spectrometry analysis of Akt1 immunoprecipitates fromglucosamine-treated cells, but not untreated controls, showed a peptide containing S473/T479 that was presumably modified withO-GlcNAc. Furthermore, in vitroO-GlcNAc-modification analysis of wildtype and mutant Akt1 revealed that S473 was targeted by recombinant OGT. A S473A Akt1 mutant demonstrated reduced basal and glucosamine-induced Akt1 O-GlcNAc modification compared with wildtype Akt1. Furthermore, wildtype Akt1, but not the S473A mutant, appeared to be associated with OGT following glucosamine treatment. Together, these observations suggest that Akt1 Ser473 may undergo both phosphorylation and O-GlcNAc modification, and the balance between these may regulatemurine β-pancreatic cell fate. | en |
dc.language.iso | en | - |
dc.publisher | Elsevier | en |
dc.subject | Apoptosis | en |
dc.subject | Akt | en |
dc.subject | Posttranslational modification | en |
dc.subject | Phosphorylation | en |
dc.subject | O-glycosylation | en |
dc.title | O-GlcNAc modulation at Akt1 Ser473 correlates with apoptosis of murine pancreatic β cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이정원 | - |
dc.contributor.AlternativeAuthor | 강은실 | - |
dc.contributor.AlternativeAuthor | 한도현 | - |
dc.contributor.AlternativeAuthor | 박정은 | - |
dc.contributor.AlternativeAuthor | 곽태경 | - |
dc.contributor.AlternativeAuthor | 오민아 | - |
dc.contributor.AlternativeAuthor | 이신애 | - |
dc.contributor.AlternativeAuthor | 최수용 | - |
dc.contributor.AlternativeAuthor | 박지용 | - |
dc.contributor.AlternativeAuthor | 김영수 | - |
dc.identifier.doi | 10.1016/j.yexcr.2008.04.014 | - |
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