Browse

Effects of sevoflurane on the cAMP-induced short-circuit current in mouse tracheal epithelium and recombinant Cl- (CFTR) and K+ (KCNQ1) channels

Cited 6 time in Web of Science Cited 10 time in Scopus
Authors
Kim, J. K.; Yoo, H. Y.; Kim, S. J.; Hwang, Y. S.; Han, J.; Kim, J. A.; Kim, C. S.; Cho, H. S.
Issue Date
2007-06-15
Publisher
Oxford University Press
Citation
Br J Anaesth. 2007 Aug;99(2):245-51. Epub 2007 Jun 13.
Keywords
Anesthetics, Inhalation/*pharmacologyAnimalsCells, CulturedChlorides/metabolismCyclic AMP/pharmacologyCystic Fibrosis Transmembrane Conductance Regulator/*drugeffects/metabolismDiffusion Chambers, CultureFemaleKCNQ1 Potassium Channel/*antagonists & inhibitors/metabolismMaleMethyl Ethers/*pharmacologyMicePatch-Clamp TechniquesPotassium Channel Blockers/pharmacologyRespiratory Mucosa/drug effects/metabolismTrachea/*drug effects/metabolism
Abstract
BACKGROUND: An optimal level of airway surface liquid is essential for mucociliary clearance in lungs. The cAMP-activated cystic fibrosis transmembrane conductance regulator (CFTR) and KCNQ1 channels in tracheal epithelium play key roles in luminal and basolateral membranes, respectively. The aim of this study was to examine the effects of sevoflurane on cAMP-induced chloride secretion by the mouse tracheal epithelium and the modulation of recombinant CFTR and KCNQ1 channels. METHODS: The equivalent short-circuit current (Isc) of the mouse tracheal epithelium was measured using a flow-type Ussing chamber technique. Inhibition of Na+ absorption was achieved through the luminal application of amiloride. cAMP-dependent Cl- secretion was evoked by forskolin and isobutylmethylxanthine (Fsk/IBMX) applied to the basolateral side. The effect of sevoflurane on CFTR and KCNQ1 channels was assessed using a whole-cell patch clamp in human embryonic kidney 293T cells expressing CFTR and KCNQ1 channels. RESULTS: Fsk/IBMX induced a sustained Isc that was suppressed by the application of sevoflurane [decreased by 49 (4.5)% at 190 microM]. The Fsk/IBMX-induced Isc was also blocked by basolateral application of chromanol 293B, a blocker of the KCNQ1 K+ channel. In KCNQ1-expressing cells, sevoflurane 190 microM reduced the outward currents to 59 (4.9)% at 80 mV. The CFTR current was not affected by sevoflurane (approximately 360 microM). CONCLUSIONS: These results suggest that the inhibition of KCNQ1 underlies sevoflurane-induced decrease in airway secretion.
ISSN
0007-0912 (Print)
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17567648
Language
English
URI
https://hdl.handle.net/10371/36653
DOI
https://doi.org/10.1093/bja/aem123
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse