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Phosphorylation of hepatitis B virus Cp at Ser87 facilitates core assembly

Cited 26 time in Web of Science Cited 28 time in Scopus
Authors
Kang, Hee Yong; Lee, Seungkeun; Park, Sung Gyoo; Yu, Jaehoon; Kim, Youngsoo; Jung, Guhung
Issue Date
2006-06-03
Publisher
Portland Press
Citation
Biochem J. 2006 Sep 1;398(2):311-7.
Keywords
Amino Acid SequenceCapsid Proteins/chemistry/genetics/*metabolism/ultrastructureCell Line, TumorCyclic AMP-Dependent Protein Kinases/metabolismHepatitis B virus/*chemistry/classification/*metabolism/ultrastructureHumansKineticsMicroscopy, Electron, TransmissionMolecular Sequence DataPhosphorylationProtein BindingSerine/genetics/*metabolismSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSurface Plasmon Resonance*Virus Assembly
Abstract
Protein-protein interactions can be regulated by protein modifications such as phosphorylation. Some of the phosphorylation sites (Ser155, Ser162 and Ser170) of HBV (hepatitis B virus) Cp have been discovered and these sites are implicated in the regulation of viral genome encapsidation, capsid localization and nucleocapsid maturation. In the present report, the dimeric form of HBV Cp was phosphorylated by PKA (protein kinase A), but not by protein kinase C in vitro, and the phosphorylation of dimeric Cp facilitated HBV core assembly. Matrix-assisted laser-desorption ionization-time-of-flight analysis revealed that the HBV Cp was phosphorylated at Ser87 by PKA. This was further confirmed using a mutant HBV Cp with S87G mutation. The S87G mutation inhibited the phosphorylation and, as a result, the in vitro HBV core assembly was not facilitated by PKA. In addition, when either pCMV/FLAG-Core(WT) or pCMV/FLAG-Core(S87G) was transfected into HepG2 cells, few mutant Cps (S87G) assembled into capsids compared with the wild-type (WT) Cps, although the same level of total Cps was expressed in both cases. In conclusion, PKA facilitates HBV core assembly through phosphorylation of the HBV Cp at Ser87.
ISSN
1470-8728 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16740137

http://hdl.handle.net/10371/38232
DOI
https://doi.org/10.1042/BJ20060347
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College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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