Browse

Light-activated indole-3-acetic acid induces apoptosis in g361 human melanoma cells

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Kim, Dong-Seok; Kim, So-Young; Jeong, Yun-Mi; Jeon, Sang-Eun; Kim, Myo-Kyoung; Kwon, Sun-Bang; Na, Jung-Im; Park, Kyoung-Chan
Issue Date
2006-12-05
Publisher
Pharmaceutical Society of Japan
Citation
Biol Pharm Bull. 2006 Dec;29(12):2404-9.
Keywords
Apoptosis/*drug effectsBlotting, WesternCell Line, TumorCold TemperatureFlow CytometryHumansIndoleacetic Acids/*pharmacology*LightLipid PeroxidationMelanoma/metabolism/*pathologyReactive Oxygen Species/metabolism
Abstract
Indole-3-acetic acid (IAA) activation by horseradish peroxidase (HRP) has been suggested as a new cancer therapy. Interestingly, we found that ultraviolet B UVB radiation also can activate IAA and produce free radicals in a dose-dependent manner. In this study, we attempted to identify the free radicals generated by UVB-irradiated IAA (IAAUVB), and to determine whether IAAUVB can induce the apoptosis of G361 human melanoma cells. Since IAA/HRP produces reactive oxygen species (ROS), we examined whether IAAUVB-generated radicals include ROS. Our results show that IAAUVB-induced free radical production is not inhibited by catalase, superoxide dismutase, or sodium formate, indicating that ROS are not generated by IAAUVB. On the other hand, IAAUVB caused lipid peroxidation, and this was blocked by Trolox, a water-soluble vitamin E derivative. Moreover, we found that IAAUVB caused apoptotic cell death and that this was inhibited by a low temperature. We further investigated IAAUVB-mediated apoptotic pathways, and found that IAAUVB causes caspase-8, Bid, caspase-3 activation, and poly (ADP-ribose) polymerase (PARP) cleavage. In addition, these apoptotic pathways were also blocked by low temperature. From these results, we propose that IAAUVB-induced free radicals cause human melanoma cell apoptosis via a death receptor-mediated apoptotic pathway.
ISSN
0918-6158 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17142972

http://hdl.handle.net/10371/39189
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse