S-Space College of Medicine/School of Medicine (의과대학/대학원) Molecular and Genomic Medicine (분자유전체의학전공) Journal Papers (저널논문_분자유전체의학전공)
Indole-3-acetic acid/horseradish peroxidase-induced apoptosis involves cell surface CD95 (Fas/APO-1) expression
- Kim, Dong-Seok; Kim, So-Young; Jeong, Yun-Mi; Jeon, Sang-Eun; Kim, Myo-Kyoung; Kwon, Sun-Bang; Park, Kyoung-Chan
- Issue Date
- Pharmaceutical Society of Japan
- Biol Pharm Bull. 2006 Aug;29(8):1625-9.
- Antibodies/immunology; Antigens, CD95/immunology/*metabolism; Apoptosis/*drug effects; Blotting, Western; Catalase/metabolism; Cell Line, Tumor; Cell Membrane/metabolism; Flow Cytometry; Horseradish Peroxidase/*pharmacology; Humans; Indoleacetic Acids/*pharmacology; Microscopy, Confocal
- Recently, we showed that a combination of indole-3-acetic acid (IAA) and horseradish peroxidase (HRP) produces hydrogen peroxide (H2O2), and that this leads to the apoptosis of G361 human melanoma cells. In the present study, flow cytometric analysis confirmed that H2O2 is involved the IAA/HRP-induced apoptotic process. We also found that IAA/HRP increases cell surface CD95 (Fas/APO-1) expression, and that this is blocked by catalase treatment. Furthermore, blocking CD95 with a neutralizing antibody significantly restored IAA/HRP-induced apoptosis. In addition, the IAA/HRP-induced activations of CD95 downstream molecules, i.e., caspase-8, Bid, and caspase-3, were also inhibited by catalase. Moreover, a caspase-8 inhibitor significantly blocked IAA/HRP-induced apoptosis. These results indicate that IAA/HRP-induced apoptosis involves a CD95-initiated death receptor signaling pathway initiated by hydrogen peroxide.
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