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Sphingosylphosphorylcholine-induced ERK activation inhibits melanin synthesis in human melanocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dong-Seok | - |
dc.contributor.author | Park, Seo-Hyoung | - |
dc.contributor.author | Kwon, Sun-Bang | - |
dc.contributor.author | Park, Eun-Sang | - |
dc.contributor.author | Huh, Chang-Hun | - |
dc.contributor.author | Youn, Sang-Woong | - |
dc.contributor.author | Park, Kyoung-Chan | - |
dc.date.accessioned | 2010-01-20T02:30:18Z | - |
dc.date.available | 2010-01-20T02:30:18Z | - |
dc.date.issued | 2006-03-10 | - |
dc.identifier.citation | Pigment Cell Res. 2006 Apr;19(2):146-53. | en |
dc.identifier.issn | 0893-5785 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16524430 | - |
dc.identifier.uri | https://hdl.handle.net/10371/39191 | - |
dc.description.abstract | Sphingosylphosphorylcholine (SPC) is emerging as a potent signaling-lipid mediator. In this study, we investigated the effects of SPC on melanogenesis using cultured human melanocytes. Our results show that SPC significantly inhibits melanin synthesis in a concentration-dependent manner, and further that it reduces the activity of tyrosinase, the rate-limiting melanogenic enzyme. SPC treatment was also found to induce short-thick dendrites in human melanocytes, but not to reduce tyrosinase activity in a cell-free system, whereas kojic acid directly inhibited tyrosinase. These results suggest that SPC reduces pigmentation by indirectly regulating tyrosinase. In further experiments, SPC was found to downregulate microphthalmia-associated transcription factor (MITF) and tyrosinase, and Western blotting showed that SPC induces the activations of extracellular signal-regulated kinase (ERK) and 90 kDa ribosomal S6 kinase (RSK-1). Moreover, the specific ERK pathway inhibitor, PD98059, blocked the hypopigmentation effect of SPC, and abrogated the SPC-mediated downregulation of MITF. These results suggest that the ERK pathway is involved in the melanogenic signaling cascade, and that ERK activation by SPC reduces melanin synthesis via MITF downregulation. | en |
dc.language.iso | en | - |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Antioxidants/pharmacology | en |
dc.subject | Cells, Cultured | en |
dc.subject | Dose-Response Relationship, Drug | en |
dc.subject | Down-Regulation/drug effects/physiology | en |
dc.subject | Enzyme Activation/drug effects/physiology | en |
dc.subject | Extracellular Signal-Regulated MAP Kinases/metabolism | en |
dc.subject | Flavonoids/pharmacology | en |
dc.subject | Humans | en |
dc.subject | MAP Kinase Signaling System/*drug effects/physiology | en |
dc.subject | Male | en |
dc.subject | Melanins/*biosynthesis | en |
dc.subject | Melanocytes/cytology/*physiology | en |
dc.subject | Microphthalmia-Associated Transcription Factor/biosynthesis | en |
dc.subject | Monophenol Monooxygenase/antagonists & inhibitors/*metabolism | en |
dc.subject | Phosphorylcholine/*analogs & derivatives/metabolism/pharmacology | en |
dc.subject | Pigmentation/drug effects/*physiology | en |
dc.subject | Pyrones/pharmacology | en |
dc.subject | Ribosomal Protein S6 Kinases, 90-kDa/metabolism | en |
dc.subject | Sphingosine/*analogs & derivatives/metabolism/pharmacology | en |
dc.title | Sphingosylphosphorylcholine-induced ERK activation inhibits melanin synthesis in human melanocytes | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김동석 | - |
dc.contributor.AlternativeAuthor | 박서형 | - |
dc.contributor.AlternativeAuthor | 권선방 | - |
dc.contributor.AlternativeAuthor | 박은상 | - |
dc.contributor.AlternativeAuthor | 허창훈 | - |
dc.identifier.doi | 10.1111/j.1600-0749.2005.00287.x | - |
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