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Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

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dc.contributor.authorKim, Kyunggon-
dc.contributor.authorOh, Jongkil-
dc.contributor.authorHan, Dohyun-
dc.contributor.authorKim, Eunice EunKyeong-
dc.contributor.authorLee, Byungil-
dc.contributor.authorKim, Youngsoo-
dc.date.accessioned2010-01-20T02:32:41Z-
dc.date.available2010-01-20T02:32:41Z-
dc.date.issued2006-01-13-
dc.identifier.citationBiochem Biophys Res Commun. 2006 Feb 24;340(4):1028-38. Epub 2005 Dec 27.en
dc.identifier.issn0006-291X (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16403447-
dc.identifier.urihttp://hdl.handle.net/10371/39195-
dc.description.abstractPilF is a requisite protein involved in the type 4 pilus biogenesis system from the Gram-negative human pathogenic bacteria, Pseudomonas aeruginosa. We determined the PilF structure at a 2.2A resolution; this includes six tandem tetratrico peptide repeat (TPR) units forming right-handed superhelix. PilF structure was similar to the heat shock protein organizing protein, which interacts with the C-terminal peptide of Hsp90 and Hsp70 via a concave Asn ladder in the inner groove of TPR superhelix. After simulated screening, the C-terminal pentapeptides of PilG, PilU, PilY, and PilZ proved to be a likely candidate binding to PilF, which are ones of 25 necessary components involved in the type 4 pilus biogenesis system. We proposed that PilF would be critical as a bridgehead in protein-protein interaction and thereby, PilF may bind a necessary molecule in type 4 pilus biogenesis system such as PilG, PilU, PilY, and PilZ.en
dc.language.isoen-
dc.publisherElsevieren
dc.subjectAmino Acid Sequenceen
dc.subjectBinding Sitesen
dc.subjectBiogenesisen
dc.subjectComputer Simulationen
dc.subjectCrystallographyen
dc.subjectDimerizationen
dc.subjectFimbriae Proteins/*chemistry/metabolism/*ultrastructureen
dc.subjectFimbriae, Bacterial/*chemistry/physiology/ultrastructureen
dc.subjectModels, Biologicalen
dc.subject*Models, Molecularen
dc.subjectMolecular Sequence Dataen
dc.subjectMultiprotein Complexes/chemistry/ultrastructureen
dc.subjectProtein Bindingen
dc.subjectProtein Conformationen
dc.subjectPseudomonas aeruginosa/*chemistry/growth & development/ultrastructureen
dc.subjectStructure-Activity Relationshipen
dc.titleCrystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosaen
dc.typeArticleen
dc.contributor.AlternativeAuthor김경곤-
dc.contributor.AlternativeAuthor오종길-
dc.contributor.AlternativeAuthor한도현-
dc.contributor.AlternativeAuthor김은경-
dc.contributor.AlternativeAuthor이병일-
dc.contributor.AlternativeAuthor김영수-
dc.identifier.doi10.1016/j.bbrc.2005.12.108-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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