Browse

Negative Feedback Regulation of Aurora-A via Phosphorylation of Fas-associated Factor-1

DC Field Value Language
dc.contributor.authorJang, Moon-Sun-
dc.contributor.authorSul, Jee-Won-
dc.contributor.authorChoi, Byung-Jung-
dc.contributor.authorLee, Su-Jin-
dc.contributor.authorSuh, Jee-Hee-
dc.contributor.authorKim, Nam-Soon-
dc.contributor.authorKim, Woo Ho-
dc.contributor.authorLim, Dae-Sik-
dc.contributor.authorLee, Chang-Woo-
dc.contributor.authorKim, Eunhee-
dc.date.accessioned2009-06-12-
dc.date.available2009-06-12-
dc.date.issued2008-09-12-
dc.identifier.citationJ Biol Chem 283, 32344-32351en
dc.identifier.issn0021-9258 (print)-
dc.identifier.issn1083-351X (online)-
dc.identifier.urihttps://hdl.handle.net/10371/4536-
dc.description.abstractThis study reports that Aurora-A (Aur-A) phosphorylates Fas-associated factor-1 (FAF1) at Ser-289 and Ser-291. Forced expression of a FAF1 mutant mimicking phosphorylation at Ser-289 and Ser-291 (FAF1 DD), but not phosphorylation-deficient FAF1 (FAF1 AA), reduced Aur-A expression. However, transfection of FAF1 DD failed to reduce Aur-A expression in the presence of MG132 and MG115, indicating that this decrease is proteasome-mediated. Additionally, transfection of FAF1 DD suppressed the expression of Aur-A in ts20-BALB cells lacking E1 ubiquitin (Ub) activating enzyme activity at restrictive temperatures and also reduced the expression of Aur-A S51D, a mutant resistant to Ub-dependent degradation. Our data indicate that phosphorylated FAF1 mediates the ubiquitin-independent, proteasome-dependent degradation of Aur-A. Overexpression of FAF1 DD blocked Aur-A-induced centrosome amplification and accumulated cells in G(2)/M phase, representing cellular phenotypes consistent with the anticipated loss of Aur-A. Collectively, our findings support the negative feedback regulation of Aur-A via phosphorylation of the death-promoting protein, FAF1, and disclose the presence of molecular cross-talk between constituents of the cell cycle and cell death machinery.en
dc.description.sponsorshipThis work was supported by FG08-21-02 of the 21C Frontier Functional Human Genome Project from the Ministry of Education, Science, and Technology in Korea and the Brain Korea 21 Project Fellowship.en
dc.language.isoen-
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen
dc.titleNegative Feedback Regulation of Aurora-A via Phosphorylation of Fas-associated Factor-1en
dc.typeArticleen
dc.contributor.AlternativeAuthor정문선-
dc.contributor.AlternativeAuthor설지원-
dc.contributor.AlternativeAuthor최병중-
dc.contributor.AlternativeAuthor이수진-
dc.contributor.AlternativeAuthor설지희-
dc.contributor.AlternativeAuthor김남순-
dc.contributor.AlternativeAuthor김우호-
dc.contributor.AlternativeAuthor임대식-
dc.contributor.AlternativeAuthor이창우-
dc.contributor.AlternativeAuthor김은희-
dc.identifier.doi10.1074/jbc.M804199200-
dc.identifier.doi10.1074/jbc.M804199200-
dc.citation.journaltitleJournal of biological chemistry-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
Files in This Item:
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse