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Effects of Structure of Rho GTPase-activating Protein DLC-1 on Cell Morphology and Migration
DC Field | Value | Language |
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dc.contributor.author | Kim, Tai Young | - |
dc.contributor.author | Healy, Kevin D. | - |
dc.contributor.author | Der, Channing J. | - |
dc.contributor.author | Sciaky, Noah | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.contributor.author | Juliano, Rudy L. | - |
dc.date.accessioned | 2010-01-28T01:22:16Z | - |
dc.date.available | 2010-01-28T01:22:16Z | - |
dc.date.created | 2020-12-21 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.citation | Journal of Biological Chemistry, Vol.283 No.47, pp.32762-32770 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 119502 | - |
dc.identifier.uri | https://hdl.handle.net/10371/45971 | - |
dc.description.abstract | DLC-1 encodes a Rho GTPase-activating protein (RhoGAP) and negative regulator of specific Rho family proteins (RhoA-C and Cdc42). DLC-1 is a multi-domain protein, with the RhoGAP catalytic domain flanked by an amino-terminal sterile alpha motif (SAM) and a carboxyl-terminal START domain. The roles of these domains in the regulation of DLC-1 function remain to be determined. We undertook a structure-function analysis involving truncation and missense mutants of DLC-1. We determined that the amino-terminal SAM domain functions as an autoinhibitory domain of intrinsic RhoGAP activity. Additionally, we determined that the SAM and START domains are dispensable for DLC-1 association with focal adhesions. We then characterized several mutants for their ability to regulate cell migration and identified constitutively activated and dominant negative mutants of DLC-1. We report that DLC-1 activation profoundly alters cell morphology, enhances protrusive activity, and can increase the velocity but reduce directionality of cell migration. Conversely, the expression of the amino-terminal domain of DLC-1 acts as a dominant negative and profoundly inhibits cell migration by displacing endogenous DLC-1 from focal adhesions. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.title | Effects of Structure of Rho GTPase-activating Protein DLC-1 on Cell Morphology and Migration | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
dc.identifier.doi | 10.1074/jbc.M800617200 | - |
dc.citation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.wosid | 000260893700065 | - |
dc.identifier.scopusid | 2-s2.0-57749113537 | - |
dc.citation.endpage | 32770 | - |
dc.citation.number | 47 | - |
dc.citation.startpage | 32762 | - |
dc.citation.volume | 283 | - |
dc.identifier.sci | 000260893700065 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Bang, Yung-Jue | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HEPATOCELLULAR-CARCINOMA | - |
dc.subject.keywordPlus | P190 RHOGAP | - |
dc.subject.keywordPlus | PLC-DELTA(1)-BINDING PROTEIN | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR | - |
dc.subject.keywordPlus | FOCAL ADHESIONS | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | BINDING | - |
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