Publications
Detailed Information
Functional and Clinical Evidence for NDRG2 as a Candidate Suppressor of Liver Cancer Metastasis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Dong Chul | - |
dc.contributor.author | Kang, Yun Kyung | - |
dc.contributor.author | Kim, Woo Ho | - |
dc.contributor.author | Jang, Ye Jin | - |
dc.contributor.author | Kim, Dong Joon | - |
dc.contributor.author | Park, In Young | - |
dc.contributor.author | Sohn, Bo Hwa | - |
dc.contributor.author | Sohn, Hyun Ahm | - |
dc.contributor.author | Lee, Hee Gu | - |
dc.contributor.author | Lim, Jong Seok | - |
dc.contributor.author | Kim, Jae Wha | - |
dc.contributor.author | Song, Eun Young | - |
dc.contributor.author | Kim, Dong Min | - |
dc.contributor.author | Lee, Mi-Ni | - |
dc.contributor.author | Oh, Goo Taeg | - |
dc.contributor.author | Kim, Soo Jung | - |
dc.contributor.author | Park, Kyung Chan | - |
dc.contributor.author | Yoo, Hyang Sook | - |
dc.contributor.author | Choi, Jong Young | - |
dc.contributor.author | Yeom, Young Il | - |
dc.date.accessioned | 2009-06-12T07:11:52Z | - |
dc.date.available | 2009-06-12T07:11:52Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Cancer Res 2008;68:4210-20 | en |
dc.identifier.issn | 0008-5472 (print) | - |
dc.identifier.issn | 1538-7445 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/4602 | - |
dc.description.abstract | We searched for potential suppressors of tumor metastasis by identifying the genes that are frequently down-regulated in hepatocellular carcinomas (HCC) while being negatively correlated with clinical parameters relevant to tumor metastasis, and we report here on the identification of N-myc downstream regulated gene 2 (NDRG2) as a promising candidate. NDRG2 expression was significantly reduced in HCC compared with nontumor or normal liver tissues [87.5% (35 of 40) and 62% (62 of 100) at RNA and protein levels, respectively]. Reduction of NDRG2 expression was intimately associated with promoter hypermethylation because its promoter region was found to carry extensively methylated CpG sites in HCC cell lines and primary tumors. Immunohistochemical analysis of NDRG2 protein in 100 HCC patient tissues indicated that NDRG2 expression loss is significantly correlated with aggressive tumor behaviors such as late tumor-node-metastasis (TNM) stage (P = 0.012), differentiation grade (P = 0.024), portal vein thrombi (P = 0.011), infiltrative growth pattern (P = 0.015), nodal/distant metastasis (P = 0.027), and recurrent tumor (P = 0.021), as well as shorter patient survival rates. Ectopically expressed NDRG2 suppressed invasion and migration of a highly invasive cell line, SK-Hep-1, and experimental tumor metastasis in vivo, whereas small interfering RNA-mediated knockdown resulted in increased invasion and migration of a weakly invasive cell line, PLC/PRF/5. In addition, NDRG2 could antagonize transforming growth factor beta1-mediated tumor cell invasion by specifically down-regulating the expression of matrix metalloproteinase 2 and laminin 332 pathway components, with concomitant suppression of Rho GTPase activity. These results suggest that NDRG2 can inhibit extracellular matrix-based, Rho-driven tumor cell invasion and migration and thereby play important roles in suppressing tumor metastasis in HCC. | en |
dc.description.sponsorship | Grant-in-Aid for the 21C Frontier Human Genome Functional Analysis Project from the Ministry of Science and Technology of Korea and by Korea Research Institute of Bioscience and Biotechnology Research Initiative Program. | en |
dc.language.iso | en | - |
dc.publisher | American Association for Cancer Research | en |
dc.subject | NDRG2 | en |
dc.subject | HCC | en |
dc.subject | Metastasis suppressor | en |
dc.subject | ECM | en |
dc.subject | transforming growth factor β | en |
dc.title | Functional and Clinical Evidence for NDRG2 as a Candidate Suppressor of Liver Cancer Metastasis | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이동철 | - |
dc.contributor.AlternativeAuthor | 강윤경 | - |
dc.contributor.AlternativeAuthor | 김우호 | - |
dc.contributor.AlternativeAuthor | 장예진 | - |
dc.contributor.AlternativeAuthor | 김동준 | - |
dc.contributor.AlternativeAuthor | 박인영 | - |
dc.contributor.AlternativeAuthor | 손보화 | - |
dc.contributor.AlternativeAuthor | 손현암 | - |
dc.contributor.AlternativeAuthor | 이희구 | - |
dc.contributor.AlternativeAuthor | 임종석 | - |
dc.contributor.AlternativeAuthor | 김재화 | - |
dc.contributor.AlternativeAuthor | 송은영 | - |
dc.contributor.AlternativeAuthor | 김동민 | - |
dc.contributor.AlternativeAuthor | 이미니 | - |
dc.contributor.AlternativeAuthor | 오구택 | - |
dc.contributor.AlternativeAuthor | 김수정 | - |
dc.contributor.AlternativeAuthor | 박경찬 | - |
dc.contributor.AlternativeAuthor | 유향숙 | - |
dc.contributor.AlternativeAuthor | 최종영 | - |
dc.contributor.AlternativeAuthor | 염영일 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-07-5040 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-07-5040 | - |
dc.citation.journaltitle | Cancer research | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.