S-Space College of Medicine/School of Medicine (의과대학/대학원) Internal Medicine (내과학전공) Journal Papers (저널논문_내과학전공)
Anti-allergic effects of PG102, a water-soluble extract prepared from Actinidia arguta, in a murine ovalbumin-induced asthma model
- Kim, D; Kim, S H; Park, E-J; Kang, C-Y; Cho, S-H; Kim, S
- Issue Date
- Clin Exp Allergy. 2009 Feb;39(2):280-9. Epub 2008 Nov 17.
- Actinidia/*chemistry; Animals; Asthma/chemically induced/*drug therapy/immunology/physiopathology; B-Lymphocytes/drug effects; Bronchial Hyperreactivity/physiopathology; Bronchoalveolar Lavage Fluid/chemistry/cytology; Cytokines/analysis/metabolism; *Disease Models, Animal; Eosinophils/cytology; Female; Forkhead Transcription Factors/genetics; Fruit/chemistry; Gene Expression/drug effects/genetics; Heme Oxygenase-1/genetics/metabolism; Humans; Immunoglobulin E/blood/metabolism; Interleukin-10/genetics; Lung/drug effects/metabolism/pathology; Mast Cells/drug effects/metabolism; Mice; Mice, Inbred BALB C; Ovalbumin/immunology; Phytotherapy/*methods; Plant Extracts/pharmacology/*therapeutic use; Respiratory Function Tests; T-Lymphocytes/drug effects/metabolism; Transforming Growth Factor beta1/genetics
- BACKGROUND: Asthma is a chronic inflammatory disease of the lung and its incidence has been increasing around the world. We previously reported that oral administration of a water-soluble extract prepared from Actinidia arguta, code-named PG102, could modulate the level of Th1 and Th2 cytokines and suppress the production of immunoglobulin E (IgE) in the ovalbumin (OVA)-immunized murine model as well as in the in vitro cell culture system, and furthermore could significantly improve dermatitis conditions in the NC/Nga murine model. These data suggested that PG102 might have therapeutic effects in a broad range of allergic diseases. OBJECTIVE: To assess the possible anti-allergic effects of PG102 in the OVA-induced murine asthma model. METHODS: The quality of PG102 was standardized, using its effects on the production of IgE, IL-5, and IL-13, in in vitro cell culture systems. To test effects on asthma, BALB/c mice were orally administrated with PG102, followed by OVA sensitization and challenge to induce asthmatic symptoms. Airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid, serum, and lung tissue were analysed by using various methods. RESULTS: PG102 could decrease the level of IgE, IL-5, and IL-13 in in vitro cell culture systems with IC(50) being 1.12-1.43 mg/mL. PG102 could ameliorate asthmatic symptoms, including AHR and eosinophilia in the lungs. Such improvement of asthmatic symptoms by PG102 was accompanied by the down-regulation of IL-5 and IgE. In PG102-treated mice, high level expression of heme oxygenase-1, a potent anti-inflammatory enzyme, was observed in alveolar inflammatory cells, while the mRNA levels of foxp3, TGF-beta1, and IL-10, important markers for regulatory T cells, were also up-regulated in the lung tissue. CONCLUSIONS: PG102 may have potential as a safe and effective reagent for the prevention or treatment of asthma.
- 1365-2222 (Electronic)
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