S-Space College of Medicine/School of Medicine (의과대학/대학원) Molecular and Genomic Medicine (분자유전체의학전공) Journal Papers (저널논문_분자유전체의학전공)
Differential effects of secreted frizzled-related proteins (sFRPs) on osteoblastic differentiation of mouse mesenchymal cells and apoptosis of osteoblasts
- Cho, Sun Wook; Her, Sun Ju; Sun, Hyun Jin; Choi, Ok Kyong; Yang, Jae-Yeon; Kim, Sang Wan; Kim, Seong Yeon; Shin, Chan Soo
- Issue Date
- Biochem Biophys Res Commun. 2008 Mar 7;367(2):399-405. Epub 2007 Dec 31.
- Animals; Apoptosis/physiology; Cell Differentiation/physiology; Cell Line; Glycoproteins/*metabolism; Membrane Proteins/*metabolism; Mesenchymal Stem Cells/*cytology/*physiology; Mice; Mice, Inbred C3H; Osteoblasts/*cytology/*physiology; Proto-Oncogene Proteins/*metabolism
- Secreted frizzled-related proteins (sFRPs) are modulators of Wnt signaling. This study was undertaken for definitive assessment of contribution of different sFRPs in osteoblastic differentiation of mesenchymal progenitor cells and apoptosis of osteoblasts. Treatment of C3H10T1/2 cells with sFRP-2 at concentrations of 10, 50, and 100nM and sFRP-4 at low concentrations (5nM) significantly increased Wnt-3A-induced alkaline phosphatase (ALP) activities, whereas sFRP-1 or 3 did not. Retroviral transduction of the sFRP-2 but not other sFRPs also significantly enhanced ALP activity induced by beta-glycerophosphate and ascorbic acid. Furthermore, transfection of all the sFRP expression vectors significantly increased beta-catenin/TCF reporter activity and the effects were most prominent with sFRP-2 and -4. In osteoblast apoptosis assay, only sFRP-3 increased etoposide-induced apoptosis in MC3T3-E1 mouse osteoblasts. In conclusion, we found that different repertoires of sFRPs exert differential effects on osteoblastic differentiation of mouse mesenchymal cells and cellular apoptosis of mouse osteoblasts in vitro.
- 1090-2104 (Electronic)