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Myocardial SSAT induction via AMPK signaling and its implication for ischemic injury

Cited 16 time in Web of Science Cited 17 time in Scopus
Authors
Ryu, Ji-Hye; Cho, Young-Suk; Chun, Yang-Sook; Park, Jong-Wan
Issue Date
2007-12-08
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2008 Feb 8;366(2):438-44. Epub 2007 Dec 4.
Keywords
Acetyltransferases/*metabolismAnimalsApoptosisCells, CulturedMaleMyocardial Reperfusion Injury/*metabolism/*pathologyMyocytes, Cardiac/*metabolism/*pathologyProtein Kinases/*metabolismRatsRats, Sprague-DawleySignal Transduction
Abstract
Spermidine/spermine N-1-acetyl-transferase (SSAT) is a catabolic enzyme that participates in polyamine metabolism. SSAT has been reported to be induced in some organs subjected to ischemia-reperfusion, but its induction mechanism has not been clarified, and little is known about SSAT regulation by ischemia per se. We induced regional ischemia of rat heart by coronary ligation and found that SSAT expression increased in ischemic myocardium. In neonatal rat cardiomyocytes and HEK293 cells, SSAT was up-regulated at the transcriptional step primarily by ATP depletion rather than oxygen deprivation. Moreover, an AMPK inhibitor compound C and AMPKalpha1-silencing RNAs attenuated the SSAT induction by ATP depletion, and an AMPK activator AICAR induced SSAT expression even without ATP depletion. When SSAT was suppressed using siRNA, the caspase activities and Bax/Bcl-2 ratios further increased in ATP depletion. These results suggest that myocardial SSAT is induced by AMPK signaling and function as a cardioprotectant under ATP-depleted conditions.
ISSN
1090-2104 (Electronic)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4R8P455-7&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a45d410fd102cfe7bde2269c418c5a4e

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18062919

http://hdl.handle.net/10371/46693
DOI
https://doi.org/10.1016/j.bbrc.2007.11.134
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College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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