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Reactive oxygen species produced by NADPH oxidase, xanthine oxidase, and mitochondrial electron transport system mediate heat shock-induced MMP-1 and MMP-9 expression

Cited 105 time in Web of Science Cited 118 time in Scopus
Authors
Shin, Mi Hee; Moon, Young Ji; Seo, Jo-Eun; Lee, Youngae; Kim, Kyu Han; Chung, Jin Ho
Issue Date
2007-11-27
Publisher
Elsevier
Citation
Free Radic Biol Med. 2008 Feb 15;44(4):635-45. Epub 2007 Nov 12.
Keywords
Acetylcysteine/pharmacologyCatalase/pharmacologyCells, Cultured*Electron Transport*Hot TemperatureHumansMatrix Metalloproteinase 1/*geneticsMatrix Metalloproteinase 9/*geneticsMitochondria/*metabolismMitogen-Activated Protein Kinases/physiologyNADPH Oxidase/*physiologyRNA, Messenger/analysisReactive Oxygen Species/*metabolismSuperoxides/metabolismXanthine Oxidase/*physiology
Abstract
In addition to ultraviolet radiation, human skin is also exposed to infrared radiation (IR) from natural sunlight. IR typically increases the skin temperature. This study examined whether or not heat shock-induced ROS stimulates MMPs in keratinocyte HaCaT cells. In HaCaT cells, heat shock was found to increase the intracellular ROS levels, including hydrogen peroxide and superoxide. The heat shock treatment induced MMP-1 and MMP-9, but not MMP-2, at the mRNA and protein levels. Moreover, heat shock caused the rapid activation of the three distinct MAPKs, ERK, JNK, and p38 kinase. The heat shock-induced expression of MMP-1 and MMP-9 was significantly suppressed by a pretreatment with the antioxidant NAC or catalase. On the other hand, SOD inhibited heat shock-induced activity of MMP-9 induction, but not MMP-1. A pretreatment with NAC or catalase, but not SOD, attenuated the phosphorylation of ERK, JNK, and p38 kinase by heat shock. The potential sites of ROS generation by heat shock along with its role in the heat shock-induced expression of MMP-1 and MMP-9 were next analyzed. These results indicate that heat shock-induced ROS is promoted via NADPH oxidase, xanthine oxidase, and mitochondria. Indeed, the NADPH oxidase and xanthine oxidase activities were increased by heat shock. Overall, the ROS produced by heat shock may play an important role in the heat shock-induced activation of MAPKs, which can induce MMP-1 and-9 expressions.
ISSN
0891-5849 (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T38-4R40SKH-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=48180e206a9fd306cfa7ea2a997df4bc

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18036352

https://hdl.handle.net/10371/46696
DOI
https://doi.org/10.1016/j.freeradbiomed.2007.10.053
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College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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