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Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Kyung-Hee | - |
dc.contributor.author | Choi, Jin-Sung | - |
dc.contributor.author | Kim, Il-Jin | - |
dc.contributor.author | Ku, Ja-Lok | - |
dc.contributor.author | Park, Jae-Gahb | - |
dc.date.accessioned | 2010-01-29T15:22:15Z | - |
dc.date.available | 2010-01-29T15:22:15Z | - |
dc.date.issued | 2006-09-29 | - |
dc.identifier.citation | World J Gastroenterol. 2006 Sep 21;12(35):5651-7. | en |
dc.identifier.issn | 1007-9327 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17007017 | - |
dc.identifier.uri | https://hdl.handle.net/10371/47150 | - |
dc.description.abstract | AIM: To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS: We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR (MS-PCR), as well as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tissues. RESULTS: Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respectively. Subsequent to sodium bisulfite modification and MS-PCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each. Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77%, respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION: The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas. | en |
dc.language.iso | en | en |
dc.subject | Antigens, Neoplasm/*genetics/metabolism | en |
dc.subject | Antimetabolites, Antineoplastic/pharmacology | en |
dc.subject | Azacitidine/analogs & derivatives/pharmacology | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Colorectal Neoplasms/*genetics/metabolism/pathology | en |
dc.subject | DNA, Neoplasm/genetics/metabolism | en |
dc.subject | Gene Expression Regulation, Neoplastic/genetics | en |
dc.subject | Humans | en |
dc.subject | Neoplasm Proteins/*genetics/metabolism | en |
dc.subject | Promoter Regions, Genetic/*genetics | en |
dc.subject | RNA, Messenger/genetics/metabolism | en |
dc.subject | Stomach Neoplasms/genetics/metabolism/pathology | en |
dc.subject | DNA Methylation | - |
dc.title | Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김경희 | - |
dc.contributor.AlternativeAuthor | 최진성 | - |
dc.contributor.AlternativeAuthor | 김일진 | - |
dc.contributor.AlternativeAuthor | 구자록 | - |
dc.contributor.AlternativeAuthor | 박재갑 | - |
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