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Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues

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dc.contributor.authorKim, Kyung-Hee-
dc.contributor.authorChoi, Jin-Sung-
dc.contributor.authorKim, Il-Jin-
dc.contributor.authorKu, Ja-Lok-
dc.contributor.authorPark, Jae-Gahb-
dc.date.accessioned2010-01-29T15:22:15Z-
dc.date.available2010-01-29T15:22:15Z-
dc.date.issued2006-09-29-
dc.identifier.citationWorld J Gastroenterol. 2006 Sep 21;12(35):5651-7.en
dc.identifier.issn1007-9327 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17007017-
dc.identifier.urihttps://hdl.handle.net/10371/47150-
dc.description.abstractAIM: To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS: We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR (MS-PCR), as well as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tissues. RESULTS: Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respectively. Subsequent to sodium bisulfite modification and MS-PCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each. Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77%, respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION: The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas.en
dc.language.isoenen
dc.subjectAntigens, Neoplasm/*genetics/metabolismen
dc.subjectAntimetabolites, Antineoplastic/pharmacologyen
dc.subjectAzacitidine/analogs & derivatives/pharmacologyen
dc.subjectCell Line, Tumoren
dc.subjectColorectal Neoplasms/*genetics/metabolism/pathologyen
dc.subjectDNA, Neoplasm/genetics/metabolismen
dc.subjectGene Expression Regulation, Neoplastic/geneticsen
dc.subjectHumansen
dc.subjectNeoplasm Proteins/*genetics/metabolismen
dc.subjectPromoter Regions, Genetic/*geneticsen
dc.subjectRNA, Messenger/genetics/metabolismen
dc.subjectStomach Neoplasms/genetics/metabolism/pathologyen
dc.subjectDNA Methylation-
dc.titlePromoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissuesen
dc.typeArticleen
dc.contributor.AlternativeAuthor김경희-
dc.contributor.AlternativeAuthor최진성-
dc.contributor.AlternativeAuthor김일진-
dc.contributor.AlternativeAuthor구자록-
dc.contributor.AlternativeAuthor박재갑-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Molecular and Clinical Oncology (분자종양의학전공)Journal Papers (저널논문_분자종양의학전공)
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