S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Histochemical Analysis of the Cutaneous Wound Healing in the Amphibian
양서류 피부 상처회복과정에 대한 조직화학적 분석
- Lim, Do-Sun; Jeong, Soon-Jeong; Jeong, Je-O; Park, Joo-Cheol; Kim, Heung-Joong; Moon, Myung-Jin; Jeong, Moon-Jin
- Issue Date
- 한국전자현미경학회지 34(1): 1 11,2004
- The wound healing is very complex biological processing including inflammatory, reepithelialization and matrix construction. According to the biological systematic category, the ability of the healing is very different. Generally healing ability of the lower animal group has been known more excellent compared to its higher group. Therefore, lower animals have been used as the experimental model to explore the mechanism of the wound healing or repair. To verify histochemical characteristics of the wound healing, we have used skin of the frog (Bombina orientalis) as known common amphibian. At day 1, 10, and 16, the mucous substance was very actively synthesized and strong positive by PAS and Alcian blue (pH 2.5). Day 10 after wounding, margin of the wound was gradually strong positive by PTAH staining for detection of collagen synthesis. At 3 to 6 hour and day 23 to 27, we have found the cell division was active through the MG-P staining, in which the concentration and division of DNA in nucleus was green to deep blue color.