Effects of pilocarpine on the secretory acinar cells in human submandibular glands

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Park, Kyungpyo; Li, Jinchao; Lee, Soojung; Choi, Se-Young; Lee, Sung-Joong; Oh, Seog-Bae; Lee, Jong-Ho; Chung, Sung- Chang; Kim, Joong-Soo; Lee, Jong-Heun

Issue Date
LIFE SCIENCES 79 (26), 2441-2447
PilocarpineIon channelCytoplasmic free Ca2+ concentration ([Ca2+]i)Aquaporin-5Submandibular gland(SMG)
Pilocarpine has been used as a choice of drugs for treatment of impaired salivary flow. Although considerable data are available as to the
stimulatory effect of pilocarpine on the salivary secretion in human, its underlying mechanism, at the cellular level, has not been rigorously
studied. In this experiment, we studied the effect of pilocarpine on the ion channel activity, cytoplasmic free Ca2+ concentration ([Ca2+]i) and
aquaporin (AQP)-5 expression, which play key roles in the secretary process and determine the capacity of fluid secretion. In human
submandibular gland (SMG) acinar cells, 10−5 M pilocarpine activated the outward rectifying-current, which was predominantly K+ selective in
the whole cell patch clamp study. The pilocarpine increased [Ca2+]i in a concentration-dependent manner in the range of 10−6 M to 10−4 M. We
found that both increases of [Ca2+]i and outward rectifying- K+ current were inhibited by 10−5 M U-73122, a specific phospholipase C inhibitor.
The magnitudes of pilocarpine-induced [Ca2+]i transients were approximately 55% lower than those with the same concentration of carbachol
(CCh). Pilocarpine also increased the amount of AQP-5 protein in the apical membrane (APM) in human SMG acinar cells. Our results suggest
that pilocarpine induce salivary secretions in human by activating K+ channels, increasing [Ca2+]i via phospholipase C dependent pathway, and
increasing AQP-5 protein expression in the APM of SMG acinar cells.
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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