Effects of polychlorinated biphenyl 19 (2,2',6-trichlorobiphenyl) on contraction, Ca2+ transient, and Ca2+ current of cardiac myocytes.

Abstract

Polychlorinated biphenyls (PCBs) have been known as serious environmental pollutants, causing developmental delays, motor dysfunction, and decrease in brain dopamine level in humans and animals. We have investigated the effects of a PCB congener, 2,2 ,6-trichlorobiphenyl (PCB 19) on contractile force, Ca2+ transient, and L-type Ca2+ current (ICa,L) in guinea pig ventricular myocytes stimulated at a rate of 0.25–0.33 Hz. PCB 19 decreased contractile force in a concentration-dependent manner. During the negative inotropic response, the action potential duration at 20% (APD20), 90% of repolarization (APD90), and the action potential amplitude (APA) were decreased concentration dependently: 30 M PCB 19 reduced APD20, APD90, and APA by 36.7 ± 3.5%, 22.6 ± 3.9%, and 2.4 ± 0.6%, respectively (n 11, p < 0.01). PCB 19 30 M decreased the Ca2+ transient and the ICa,L by 46.8 ± 1.8% (n 9, p < 0.01) and 47.1 ± 3.1% (n 9, p < 0.01), respectively. The results suggest that PCB 19 decreased the Ca2+ transient through inhibition of L-type Ca2+ channels and that the decreased Ca2+ transient consequently caused a negative inotropic effect in cardiac myocytes.