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Effects of Rat Heart Endothelial Cell-Conditioned Medium on the Proliferation and Differentiation of Osteoblasts

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Authors
Kim, Gwan-shik; Lee, In-Seok; Choi, Kyu-Won; Baek, Jeong-Hwa
Issue Date
1996-06
Publisher
The Korean Academy of Oral Biology
Citation
Journal of Oral Biology. 20 14-24, 1996.
Keywords
Endothelial cellOsteoblastic cellMitogenic activityAlkaline phosphatase activity
Abstract
Bone developmnt and remodeking depend on complex interacts between bone-forming osteoblats, bone-degrading osteoclats, and other cells present within the bone mocrowironment. Espectally, bone cascular endotheliol cells may play a pivital role in these interactions via linking circulatory and local signals with cells of the bone mlcroenvironment and actovely contributing itselg to the regulation of bone cell phsiology. Thereforem the understanding of the role of endothelial cells in bone remodeling and their interactions with strimal and hematopoietic systems of bone tissue coule be very important.
In this paper, rat heart endothelial cells (RHEC) were isolated and endothelial cell-conditioned medium (ECCM) was collected from RHEC culture. The effects of ECCM on the proliferation and differentiation of osteoblast were investigated using osteoblatic cell lines and fetal calcarial cells. And the mitogenic activities were identified in partially purified ECCM.
Isolated rat heart endothelial cells had a relatively uniform morphology and exhibited a cobblestone-like appearanve, and were positively stanined with Dil-Ac-LDL. ECCM stimulated the proliferation of MG 63 and MC3T3-E1 cells, but inhibited the proliferation of ROS 17/2.8 cells. ECCM did not show any stimulatory or inhibitory effects on the alkaline phospaatase activity of MG 63 and MG3T3-E1 cells. And ECCM inhibited in vitro mineralized nodule formation by fetal rat calcarial cells. On MG 63 cells, mitogenic activities were evident in the fraction eluting close to the void colime of the column(>60 KDa) and in fractions corresponding to the molecular weight of 27, 24, 19 and 16 KDa. On Mc3T3-E1 cells, mitrogenic activity was evident only in the fration close to the void colume. However, on ROS 17/2.8 cells, growth-inhibiting activity appeared in the fraction correspinding to >60 KDa.
The demostration that osteoblastic cell lines respond to ECCM may prove to be an important contribution with respect to establishing a direct link between initiation of bone formation and cascularization.
ISSN
1225-6390
Language
English
URI
https://hdl.handle.net/10371/47242
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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