S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Effects of short-term chemical disinfection of GP cone: identification of affected microbes and alterations in surface texture and physical properties
- Kum, Kee-Yeon; Pang, Nan-Shim; Jung, Il-Young; Bae, Kwang-Shik; Baek, Seung-Ho; Lee, Woo-Cheol
- Issue Date
- JOURNAL OF ENDODONTICS 2007;33:594-598.
- The aims of this study were to identify microorganisms on the
surface of gutta-percha (GP) cones using a polymerase chain
reaction (PCR) and to evaluate the sterilization effect of
5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine
(CHX), and ChloraPrep on these bacteria. Alterations of the
surface texture and physical properties of GP cone after
chemical sterilization were compared. A total of 150 GP
cones were randomly selected in endodontic clinics, and a
PCR assay was performed to identify the contaminating microorganisms.
After inoculation with the same microorganisms
followed by drying for 1 day, the GP cones were immersed
in the three chemical disinfectants. The sterilization
effects were assessed by measuring the turbidity and through
subculturing. The changes in surface texture were observed
by scanning electron microscopy, and Instron 5500 (Instron
Corp, Canton, MA) was used to measure the tensile strength
and elongation rate of the GP cones after chemical sterilization.
Statistical analysis was performed. Results indicate that
19.4% of GP cones from the clinic were contaminated, and all
the species belonged to the genus Staphylococcus. Three
chemical disinfectants were all effective in the rapid disinfection
of GP cones against Staphylococcus spp, and 1-minute
immersion of the GP cones was adequate for disinfection.
Microphotographs of the NaOCl-soaked cone showed a cluster
of cuboidal crystals. The tensile strength between the
NaOCl- and CHX-soaked groups was significantly different (p
0.05), but there was no significant difference between the
NaOCl- and ChloraPrep-soaked groups (p 0.05). All disinfectants
significantly increased the elongation rate of the GP
cones compared with fresh GP cones (p 0.05), especially in
the ChloraPrep (Medi-flex, Leawood, KS). These results show
that the three chemical disinfectants are effective agents for
the rapid sterilization of GP cones. However, further research
will be needed to determine the clinical relevance of the
changes in physical properties. (J Endod 2007;33:594–598)
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