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법랑기질유도체가 탈회 치근표면에서 치주인대섬유아세포의 생물학적 성상에 미치는 영향

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Issue Date
2008
Publisher
대한치주과학회
Citation
대한치주과학회지 2008;38:679-690.
Keywords
Enamel matrix derivativesPDL cellTetracyclineEDTA
Abstract
Purpose: The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). Material and Methods: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done. Results: Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days. Conclusion: Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.
ISSN
0250-3352
Language
Korean
URI
http://uci.or.kr/G100:I100-KOI(KISTI1.1003/JNL.JAKO200805441026723)

https://hdl.handle.net/10371/47418
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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