구강점막 표면 잔존타액 및 Hydroxyapatite 표면 Lysozyme 활성에 관한 연구

Abstract

The residual saliva has been known to he important for the maintenance of oral mucosal wetness. Salivary proteins of the oral mucosal film function as a moisture retainer and a protective barrier. Enamel pellicles, derived from saliva, playa significant role in the maintenance and microbial colonization of tooth surfaces. The purpose of this study was to report lysozyme activity of residual saliva and to compare its activity with those of other kinds of saliva, and to investigate the activity of adsorbed lysozyme on the surface of hydroxyapatite. The stimulated and unstimulated whole saliva, stimulated parotid and submandibular-sublingual saliva, and residual saliva on buccal mucosa were collected from a healthy subject. For the investigation of adsorbed lysozyme, hen egg-white lysozyme and unstimulated whole saliva were used as sources of lysozyme. The total protein concentration of all salivary samples was determined using the ninhydrin assay following acid hydrolysis. The lysozyme activity was determined by the turbidimetric method with Micrococcus lysodeikticus. The residual saliva showed higher protein concentration and higher lysozyme activity compared to whole saliva. The residual saliva has more high-molecular-weight proteins compared to other kinds of saliva. The lysozyme displayed decreased activity on the surface of hydroxyapatite. Future additional studies on the composition and activity of both antimicrobial and non-antimicrobial salivary proteins in residual saliva and enamel pellicle are essential.