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Experimental salivary pellicles on the surface of self-curing resin

Cited 10 time in Web of Science Cited 8 time in Scopus
Authors
Kho, H.-S; Yoo, J.-H; Kim, Y.-K; Lee, S.-W; Chung, S.-C
Issue Date
2003-02
Publisher
Wiley-Blackwell
Citation
JOURNAL OF ORAL REHABILITATION. 30, 251-259.
Keywords
salivapellicleresin
Abstract
summary The aim of this study was to identify the salivary components present in the pellicles formed on self-curing resin and to investigate the qualitative variations in adsorbed salivary pellicle compositions according to different exposure time to saliva. Experimental pellicles were formed by the incubation of polymerized resin particles with fresh human parotid or submandibular-sublingual saliva for either 20 min or 2 h. Pellicles were extracted using formic acid and lyophilized, they were then subjected to sodium dodecyl sulphate–polyacrylamide gel electrophoresis and immunoblotting to identify the adsorbed salivary components. The amino acid profiles of the 2 h-pellicles were analysed and compared with those of fresh glandular salivas. There was a difference in the 2 h-pellicle components on the self-curing resin compared with those of other dental materials as well as tooth enamel. The amino acid profiles of the 2 h-pellicles were also different from those of fresh glandular salivas. In the case of submandibular-sublingual saliva, the components of the 2 h-pellicle showed a different pattern compared with those of the 20 min-pellicle. However, there was no significant difference between the components of the 2 h- and 20 min-pellicles in the case of parotid saliva. A distinct difference was found in the surface binding affinities of immunoglobulin (IgA) from different glandular salivas. The findings of this study provide information concerning the initial bacterial adhesion on the surfaces of self-curing resin.
ISSN
0305-182X
Language
English
URI
https://hdl.handle.net/10371/47516
DOI
https://doi.org/10.1046/j.1365-2842.2003.01013.x
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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