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CELLULAR RESPONSES ON ANODIZED TITANIUM DISCS COATED WITH ,25-DIHYDROXYVITAMIN D3 INCORPORATED POLY (D,L-LACTIDE-CO-GLYCOLIDE) (PLGA) NANOPARTICLES
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Young-Jin | - |
dc.contributor.author | Heo, Seong-Joo | - |
dc.contributor.author | Koak, Jai-Young | - |
dc.contributor.author | Kim, Seong-Kyun | - |
dc.contributor.author | Lee, Joo-Hee | - |
dc.date.accessioned | 2010-02-01T09:48:37Z | - |
dc.date.available | 2010-02-01T09:48:37Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | 2008;46(6) | en |
dc.identifier.issn | 0301-2875 | - |
dc.identifier.uri | https://hdl.handle.net/10371/47742 | - |
dc.description.abstract | STATEMENT OF PROBLEM: A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces. PURPOSE: The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/,25-(OH) coating with reference to cellular proliferation and differentiation in vitro. MATERIAL AND METHODS: 96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/,25-(OH) solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTSbased cell proliferation assay and ALPase activity test were carried out. RESULTS: PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated ,25-(OH) (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05). CONCLUSION: Biodegradable PLGA nanoparticles incorporated with vitamin D metabolite positively affected proliferation and differentiation of cells on the anodized titanium surface. | en |
dc.description.sponsorship | This work was supported by the Korea Research Foundation Grant Funded by the Korean Government (MOEHRD,Basic Research Promotion Fund)(KRF-2007-331-E00245). | en |
dc.language.iso | en | en |
dc.publisher | 대한치과보철학회 | en |
dc.subject | 25-dihydroxyvitamin | en |
dc.subject | PLGA | en |
dc.subject | Nanoparticle | en |
dc.subject | Anodized titanium | en |
dc.subject | Cell prolifera | en |
dc.subject | Differentiation | en |
dc.title | CELLULAR RESPONSES ON ANODIZED TITANIUM DISCS COATED WITH ,25-DIHYDROXYVITAMIN D3 INCORPORATED POLY (D,L-LACTIDE-CO-GLYCOLIDE) (PLGA) NANOPARTICLES | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 조영진 | - |
dc.contributor.AlternativeAuthor | 허성주 | - |
dc.contributor.AlternativeAuthor | 곽재영 | - |
dc.contributor.AlternativeAuthor | 김성균 | - |
dc.contributor.AlternativeAuthor | 이주희 | - |
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