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Increased association of dynamin II with myosin II in ras transformed NIH3T3 cells

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Authors
JEONG, Soon-Jeong; KIM, Su-Gwan; YOO, Jiyun; HAN, Mi-Young; PARK, Joo-Cheol; KIM, Heung-Joong; KANG, Seong Soo; CHOI, Baik-Dong; JEONG, Moon-Jin
Issue Date
2006
Publisher
Blackwell-synergy
Citation
ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2006, 38(8): 556-562
Keywords
Grb2dynamin IImyosin IIRas transformed NIH3T3 cell
Abstract
Abstract Dynamin has been implicated in the formation of nascent vesicles through both endocytic and secretory pathways. However, dynamin has recently been implicated in altering the cell membrane shape during cell migration associated with cytoskeleton-related proteins. Myosin II has been implicated in maintaining cell morphology and in cellular movement. Therefore, reciprocal immunoprecipitation was carried out to identify the potential relationship between dynamin II and myosin II. The dynamin II expression level was higher when co-expressed with myosin II in Ras transformed NIH3T3 cells than in normal NIH3T3 cells. Confocal microscopy also confirmed the interaction between these two proteins. Interestingly, exposing the NIH3T3 cells to platelet-derived growth factor altered the interaction and localization of these two proteins. The platelet-derived growth factor treatment induced lamellipodia and cell migration, and dynamin II interacted with myosin II. Grb2, a 24 kDa adaptor protein and an essential element of the Ras signaling pathway, was found to be associated with dynamin II and myosin II gene expression in the Ras transformed NIH3T3 cells. These results suggest that dynamin II acts as an intermediate messenger in the Ras signal transduction pathway leading to membrane ruffling and cell migration
ISSN
1745-7270
Language
English
URI
https://hdl.handle.net/10371/47992
DOI
https://doi.org/10.1111/j.1745-7270.2006.00193.x
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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