Publications
Detailed Information
Comparison of Agar and Agarose Culture Methods for Human Tumor Stem Cell Assay of Gynecologic Tumors : 부인과 영역의 악성종양에 있어서 human tumor stem cell assay를 위한 agar배지와 agarose배지의 비교
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Issue Date
- 1986-06
- Citation
- Seoul J Med, Vol.27 No.2, pp. 169-172
- Keywords
- Agar ; Agarose ; Clone Cells ; Gynecologic Tumor
- Abstract
- The human tumor stem cell assay (HTSCA) has broad research applications in
the field of cell biology as well as in clinical chemotherapy. A prospective clinical trial for
improvement of the in vitro growth rate of gynecologic cancers was done between agar and
agarose culture methods. Specimens from 27 gynecologic tumors were disaggregated by
mechanical and enzymatic methods and assayed in soft agar and agarose culture matrix. 9 of
27 tumors grew in the agar system yielding a cloning success rate of 33.3%. Of 27 specimens,
17 have shown colony growth in the agarose culture matrix showing a cloning success rate of
63.0%. Cloning efficiency in agarose improved approximately 2-fold as compared with that in
agar. The development of a soft agarose assay for gynecologic tumor cells will provide a
possibility of an in vitro technique for predicting in vivo response to anticancer drugs.
- ISSN
- 0582-6802
- Language
- English
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.